Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17474 | 5' | -51.3 | NC_004456.1 | + | 41254 | 0.66 | 0.857138 |
Target: 5'- uGCGGCCcACCagcuccGCACcgaguucuGCCAGCAu -3' miRNA: 3'- -CGCCGGuUGGaaua--CGUG--------UGGUUGUc -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 18951 | 0.66 | 0.857138 |
Target: 5'- aGCaGCUggAACCaug-GCGCACCAGCGu -3' miRNA: 3'- -CGcCGG--UUGGaauaCGUGUGGUUGUc -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 23725 | 0.66 | 0.839099 |
Target: 5'- cCGGgCggUgaUGUGC-CACCGGCAGa -3' miRNA: 3'- cGCCgGuuGgaAUACGuGUGGUUGUC- -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 32823 | 0.66 | 0.839099 |
Target: 5'- aUGGCCAACuCUcgccaGCGCAUCAGCu- -3' miRNA: 3'- cGCCGGUUG-GAaua--CGUGUGGUUGuc -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 14798 | 0.66 | 0.820095 |
Target: 5'- cCGGUCAccACCUUG-GCaACAUCGACAa -3' miRNA: 3'- cGCCGGU--UGGAAUaCG-UGUGGUUGUc -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 42392 | 0.67 | 0.800214 |
Target: 5'- uCGG-CAACCUgugGUGCACcguCAACAGa -3' miRNA: 3'- cGCCgGUUGGAa--UACGUGug-GUUGUC- -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 4356 | 0.69 | 0.702593 |
Target: 5'- cGCGGCCuACUUg--GUACGCCuuCAa -3' miRNA: 3'- -CGCCGGuUGGAauaCGUGUGGuuGUc -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 30592 | 0.69 | 0.691187 |
Target: 5'- aGCGGCCAAgCUgg-GCGCaggugguacauuGCC-ACAGg -3' miRNA: 3'- -CGCCGGUUgGAauaCGUG------------UGGuUGUC- -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 29975 | 0.69 | 0.656641 |
Target: 5'- gGCuGGCCAugCgg--GcCACGCCAGCAc -3' miRNA: 3'- -CG-CCGGUugGaauaC-GUGUGGUUGUc -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 22352 | 0.7 | 0.61028 |
Target: 5'- uGCGGcCCAGCC---UGCugGCUAAgGGg -3' miRNA: 3'- -CGCC-GGUUGGaauACGugUGGUUgUC- -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 35558 | 0.73 | 0.433924 |
Target: 5'- aGCGGCCAAgCgcgUGaGCGCACCcaaAGCGGc -3' miRNA: 3'- -CGCCGGUUgGa--AUaCGUGUGG---UUGUC- -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 37053 | 0.73 | 0.423838 |
Target: 5'- gGCGGCCAguugcuccgcaACCUugggcugcaaauUAUGCuCACCGGCAa -3' miRNA: 3'- -CGCCGGU-----------UGGA------------AUACGuGUGGUUGUc -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 14731 | 0.73 | 0.423838 |
Target: 5'- aGCGGCCAACCUUAU-CG-ACCAAacCAGu -3' miRNA: 3'- -CGCCGGUUGGAAUAcGUgUGGUU--GUC- -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 15208 | 0.74 | 0.404095 |
Target: 5'- aGCGGCCAGCugCUUAUGUGCAuCCGuuuauuuguGCGGa -3' miRNA: 3'- -CGCCGGUUG--GAAUACGUGU-GGU---------UGUC- -5' |
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17474 | 5' | -51.3 | NC_004456.1 | + | 15245 | 1.12 | 0.001039 |
Target: 5'- aGCGGCCAACCUUAUGCACACCAACAGa -3' miRNA: 3'- -CGCCGGUUGGAAUACGUGUGGUUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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