miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17475 5' -52 NC_004456.1 + 32680 0.66 0.817742
Target:  5'- -aGACGAaGCGUggGCCGauauuGCGGCGg -3'
miRNA:   3'- uaUUGCUaCGCG--UGGCaguu-UGCCGC- -5'
17475 5' -52 NC_004456.1 + 19729 0.67 0.745293
Target:  5'- gAUGAUGAUGCGgGCCagugauuuGUCGgccauugcGAUGGCGu -3'
miRNA:   3'- -UAUUGCUACGCgUGG--------CAGU--------UUGCCGC- -5'
17475 5' -52 NC_004456.1 + 34663 0.67 0.766813
Target:  5'- uUAGcCGGUGCGcCGCUGUUAu-UGGCGa -3'
miRNA:   3'- uAUU-GCUACGC-GUGGCAGUuuGCCGC- -5'
17475 5' -52 NC_004456.1 + 16537 0.7 0.563605
Target:  5'- uUGACGGUGCGCAUCGUUAucuuugaugaGAUGaGUGc -3'
miRNA:   3'- uAUUGCUACGCGUGGCAGU----------UUGC-CGC- -5'
17475 5' -52 NC_004456.1 + 37267 0.7 0.586347
Target:  5'- -aAGCGAgGCGCACUGgUgAAACGGuCGg -3'
miRNA:   3'- uaUUGCUaCGCGUGGC-AgUUUGCC-GC- -5'
17475 5' -52 NC_004456.1 + 15439 0.7 0.609256
Target:  5'- aGUAACGAUGCggGCugCGUCGAcagcaaccucuACGGg- -3'
miRNA:   3'- -UAUUGCUACG--CGugGCAGUU-----------UGCCgc -5'
17475 5' -52 NC_004456.1 + 16599 1.08 0.001804
Target:  5'- gAUAACGAUGCGCACCGUCAAACGGCGa -3'
miRNA:   3'- -UAUUGCUACGCGUGGCAGUUUGCCGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.