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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17478 | 3' | -55.5 | NC_004456.1 | + | 414 | 0.69 | 0.388208 |
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Target: 5'- uAUGGCUGAUuuguGGAUUGCUGGCCaGCu -3' miRNA: 3'- uUACCGGCUG----UUUAGUGACCGGgCGu -5' |
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| 17478 | 3' | -55.5 | NC_004456.1 | + | 3947 | 0.7 | 0.369866 |
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Target: 5'- -cUGGCUGAUGAAUCGCUGG-CgGUAc -3' miRNA: 3'- uuACCGGCUGUUUAGUGACCgGgCGU- -5' |
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| 17478 | 3' | -55.5 | NC_004456.1 | + | 14668 | 0.67 | 0.542634 |
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Target: 5'- -uUGGUCGAUAAGguuggcCGCUGGUuuGCc -3' miRNA: 3'- uuACCGGCUGUUUa-----GUGACCGggCGu -5' |
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| 17478 | 3' | -55.5 | NC_004456.1 | + | 19081 | 0.67 | 0.488263 |
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Target: 5'- --cGGCUGACcAAUCGUUGGCCCu-- -3' miRNA: 3'- uuaCCGGCUGuUUAGUGACCGGGcgu -5' |
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| 17478 | 3' | -55.5 | NC_004456.1 | + | 19805 | 1.06 | 0.00088 |
|
Target: 5'- cAAUGGCCGACAAAUCACUGGCCCGCAu -3' miRNA: 3'- -UUACCGGCUGUUUAGUGACCGGGCGU- -5' |
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| 17478 | 3' | -55.5 | NC_004456.1 | + | 25408 | 0.67 | 0.547077 |
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Target: 5'- -cUGGCCGACAgugcauGAaacacauaagcaucaUCACU-GCCCGCc -3' miRNA: 3'- uuACCGGCUGU------UU---------------AGUGAcCGGGCGu -5' |
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| 17478 | 3' | -55.5 | NC_004456.1 | + | 33162 | 0.66 | 0.598854 |
|
Target: 5'- aGGUGGCCGA------GCUGGCCCa-- -3' miRNA: 3'- -UUACCGGCUguuuagUGACCGGGcgu -5' |
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| 17478 | 3' | -55.5 | NC_004456.1 | + | 38486 | 0.69 | 0.378963 |
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Target: 5'- --gGGUCGGCGAGUCuuccuUUGGCCCaGCc -3' miRNA: 3'- uuaCCGGCUGUUUAGu----GACCGGG-CGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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