Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17479 | 5' | -53.8 | NC_004456.1 | + | 22737 | 0.65 | 0.716223 |
Target: 5'- cGCCUGGUUGCcgguacgacuuuugAACGGCAGaaCCAugaAGGCu -3' miRNA: 3'- -UGGACUAGUG--------------UUGUCGUCc-GGU---UCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 6868 | 0.66 | 0.708399 |
Target: 5'- cACCUGGacugaaUCACcagAGCAGCcuGCCGAuGGUg -3' miRNA: 3'- -UGGACU------AGUG---UUGUCGucCGGUU-CCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 861 | 0.66 | 0.674474 |
Target: 5'- gGCCa-GUCGCAAgAGCaAGGCCAacugcaAGGUc -3' miRNA: 3'- -UGGacUAGUGUUgUCG-UCCGGU------UCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 30250 | 0.67 | 0.651619 |
Target: 5'- cACCUGAgUCugAACAgGCAuGGCgugaUAAGGUg -3' miRNA: 3'- -UGGACU-AGugUUGU-CGU-CCG----GUUCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 42572 | 0.67 | 0.651619 |
Target: 5'- -aCUGGUCugAGCaAGCugaggaAGGCagCGAGGCa -3' miRNA: 3'- ugGACUAGugUUG-UCG------UCCG--GUUCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 32184 | 0.67 | 0.603478 |
Target: 5'- gACCUGAcUACGccgccagGCAGCAaccugcuGGCCGGuGGCu -3' miRNA: 3'- -UGGACUaGUGU-------UGUCGU-------CCGGUU-CCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 19795 | 0.68 | 0.594338 |
Target: 5'- aGCC---UCAgAAguGCAGGCCcuGGCa -3' miRNA: 3'- -UGGacuAGUgUUguCGUCCGGuuCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 36144 | 0.68 | 0.581807 |
Target: 5'- aGCCUGAauggggugauuuaUCcagaauACAAaGGCGGGUUAAGGCa -3' miRNA: 3'- -UGGACU-------------AG------UGUUgUCGUCCGGUUCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 18298 | 0.68 | 0.571595 |
Target: 5'- cACCgg--CACG--AGCAGGCCAgAGGCc -3' miRNA: 3'- -UGGacuaGUGUugUCGUCCGGU-UCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 35539 | 0.68 | 0.549067 |
Target: 5'- uACCUGAacCACAAguggaAGC-GGCCAAGcGCg -3' miRNA: 3'- -UGGACUa-GUGUUg----UCGuCCGGUUC-CG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 25690 | 0.69 | 0.515826 |
Target: 5'- gUCUGGcgaaUCACAucacCAGCAGGaCCA-GGCa -3' miRNA: 3'- uGGACU----AGUGUu---GUCGUCC-GGUuCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 41288 | 0.69 | 0.504924 |
Target: 5'- cACCaUGccaUACGGCGGguGGCCA-GGCg -3' miRNA: 3'- -UGG-ACua-GUGUUGUCguCCGGUuCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 39159 | 0.7 | 0.472844 |
Target: 5'- uCCUGGUUAUgggcuucacuGGCaAGCAGGCCGccgccaucaaagAGGCa -3' miRNA: 3'- uGGACUAGUG----------UUG-UCGUCCGGU------------UCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 16941 | 0.71 | 0.373297 |
Target: 5'- aGCCUGGg-ACAAaaaaGGCAGGCCAAuuguaagcauugcGGCg -3' miRNA: 3'- -UGGACUagUGUUg---UCGUCCGGUU-------------CCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 15931 | 0.73 | 0.31477 |
Target: 5'- aGCCUGGUCGCAGCugcccguucuaagguGCucGCCAugaAGGCa -3' miRNA: 3'- -UGGACUAGUGUUGu--------------CGucCGGU---UCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 2877 | 0.73 | 0.305981 |
Target: 5'- uACCUGGcCAUgcaGGCaaAGCAGGCCAgaacAGGCg -3' miRNA: 3'- -UGGACUaGUG---UUG--UCGUCCGGU----UCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 4254 | 0.75 | 0.241095 |
Target: 5'- aACaaGAcCAcCAGCAGCuGGCCAGGGCu -3' miRNA: 3'- -UGgaCUaGU-GUUGUCGuCCGGUUCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 20380 | 0.75 | 0.218534 |
Target: 5'- aACCUGGUggccgaagcccggggCAUGACcgccgAGCAGGUCAAGGCg -3' miRNA: 3'- -UGGACUA---------------GUGUUG-----UCGUCCGGUUCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 22269 | 0.77 | 0.172845 |
Target: 5'- uGCCUGcgguuUCAUugaaauCAGCAgGGCCAAGGCg -3' miRNA: 3'- -UGGACu----AGUGuu----GUCGU-CCGGUUCCG- -5' |
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17479 | 5' | -53.8 | NC_004456.1 | + | 24401 | 0.77 | 0.168012 |
Target: 5'- aGCCUGAUCACGuCAaaauuGCAGGCgGcAGGCu -3' miRNA: 3'- -UGGACUAGUGUuGU-----CGUCCGgU-UCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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