Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17480 | 3' | -51.2 | NC_004456.1 | + | 27821 | 0.66 | 0.878603 |
Target: 5'- cGCCAGGCa---GUCCGuuguCGGCaCg-UCGa -3' miRNA: 3'- -CGGUCUGguaaCAGGU----GCCG-GunAGC- -5' |
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17480 | 3' | -51.2 | NC_004456.1 | + | 8672 | 0.66 | 0.8778 |
Target: 5'- -aCGGcACCAUUGUUaucaagugugacgUAUGGCCucUCGg -3' miRNA: 3'- cgGUC-UGGUAACAG-------------GUGCCGGunAGC- -5' |
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17480 | 3' | -51.2 | NC_004456.1 | + | 36537 | 0.66 | 0.876187 |
Target: 5'- aGCCAGACCAggguuuaauucaUaaaccuggagagcaUaGUCagaGCGGCCAuaUUGg -3' miRNA: 3'- -CGGUCUGGU------------A--------------A-CAGg--UGCCGGUn-AGC- -5' |
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17480 | 3' | -51.2 | NC_004456.1 | + | 18366 | 0.66 | 0.870454 |
Target: 5'- uCCGGGCCAUagucuccgaUGUCCu--GCCAa-CGg -3' miRNA: 3'- cGGUCUGGUA---------ACAGGugcCGGUnaGC- -5' |
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17480 | 3' | -51.2 | NC_004456.1 | + | 32236 | 0.67 | 0.796291 |
Target: 5'- aGCCAGcugcaaggcaGCCAaa--CCACGGCCAgagCGc -3' miRNA: 3'- -CGGUC----------UGGUaacaGGUGCCGGUna-GC- -5' |
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17480 | 3' | -51.2 | NC_004456.1 | + | 6050 | 0.68 | 0.775645 |
Target: 5'- aCCGGACCAggacuaUGauaCCugGGCaCGugUCGg -3' miRNA: 3'- cGGUCUGGUa-----ACa--GGugCCG-GUn-AGC- -5' |
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17480 | 3' | -51.2 | NC_004456.1 | + | 33386 | 0.68 | 0.769321 |
Target: 5'- cGCCAGgcacugaaccgaGCCGgagUGUCCACcaaggaccuugccggGGCuCAgUCGa -3' miRNA: 3'- -CGGUC------------UGGUa--ACAGGUG---------------CCG-GUnAGC- -5' |
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17480 | 3' | -51.2 | NC_004456.1 | + | 14757 | 0.68 | 0.741305 |
Target: 5'- aUCGGcACCGUUGUCaccggcaaaccaGCGGCCAaccuuaUCGa -3' miRNA: 3'- cGGUC-UGGUAACAGg-----------UGCCGGUn-----AGC- -5' |
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17480 | 3' | -51.2 | NC_004456.1 | + | 16983 | 0.7 | 0.641779 |
Target: 5'- uUCGGGCUcUUGUUCAUGGCCAcagCa -3' miRNA: 3'- cGGUCUGGuAACAGGUGCCGGUna-Gc -5' |
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17480 | 3' | -51.2 | NC_004456.1 | + | 18490 | 0.71 | 0.595786 |
Target: 5'- uGUCAGGCCAUUaccaacggCUGCGGCCAccCa -3' miRNA: 3'- -CGGUCUGGUAAca------GGUGCCGGUnaGc -5' |
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17480 | 3' | -51.2 | NC_004456.1 | + | 17320 | 0.78 | 0.254415 |
Target: 5'- uCCAGACgGUUGUCCugGGUa--UCGa -3' miRNA: 3'- cGGUCUGgUAACAGGugCCGgunAGC- -5' |
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17480 | 3' | -51.2 | NC_004456.1 | + | 20520 | 1.13 | 0.001083 |
Target: 5'- aGCCAGACCAUUGUCCACGGCCAnUCGg -3' miRNA: 3'- -CGGUCUGGUAACAGGUGCCGGUnAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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