Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
17480 | 5' | -52.8 | NC_004456.1 | + | 32876 | 0.66 | 0.776187 |
Target: 5'- gGGCuAUCUAGuagcCCUgauuuuuaaccugguUCAugGCCUUGAaCCg -3' miRNA: 3'- -UCG-UAGGUC----GGA---------------AGUugCGGAACU-GG- -5' |
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17480 | 5' | -52.8 | NC_004456.1 | + | 12960 | 0.66 | 0.761397 |
Target: 5'- aAGCGUUCAGCaaUUAGCaCCUcaguucUGGCCg -3' miRNA: 3'- -UCGUAGGUCGgaAGUUGcGGA------ACUGG- -5' |
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17480 | 5' | -52.8 | NC_004456.1 | + | 14235 | 0.66 | 0.739788 |
Target: 5'- uGGUAUCC-GUgUUCAcGgGCCUUGGCUu -3' miRNA: 3'- -UCGUAGGuCGgAAGU-UgCGGAACUGG- -5' |
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17480 | 5' | -52.8 | NC_004456.1 | + | 21140 | 0.66 | 0.736503 |
Target: 5'- cAGCGUUCAccaccGaCCccaacucgguuacaUUCAGCGCCaUGACCg -3' miRNA: 3'- -UCGUAGGU-----C-GG--------------AAGUUGCGGaACUGG- -5' |
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17480 | 5' | -52.8 | NC_004456.1 | + | 19919 | 0.67 | 0.733208 |
Target: 5'- uGGC--CCAGCCUUCcuuggugacuugguACGCCUUGcGCUu -3' miRNA: 3'- -UCGuaGGUCGGAAGu-------------UGCGGAAC-UGG- -5' |
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17480 | 5' | -52.8 | NC_004456.1 | + | 22166 | 0.67 | 0.683925 |
Target: 5'- uGGCAccaCCAacaCCUUCuuuacCGCCUUGGCCc -3' miRNA: 3'- -UCGUa--GGUc--GGAAGuu---GCGGAACUGG- -5' |
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17480 | 5' | -52.8 | NC_004456.1 | + | 14060 | 0.68 | 0.661114 |
Target: 5'- aAGCAUCUGGCCguuUUGACGCUccaGACg -3' miRNA: 3'- -UCGUAGGUCGGa--AGUUGCGGaa-CUGg -5' |
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17480 | 5' | -52.8 | NC_004456.1 | + | 20704 | 0.68 | 0.626706 |
Target: 5'- cGGCAUCC-GCUgcCAACGCUUgugUGGCUu -3' miRNA: 3'- -UCGUAGGuCGGaaGUUGCGGA---ACUGG- -5' |
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17480 | 5' | -52.8 | NC_004456.1 | + | 36690 | 0.7 | 0.547077 |
Target: 5'- gGGCuUCCAGUCU--GAUGCaUUGACCa -3' miRNA: 3'- -UCGuAGGUCGGAagUUGCGgAACUGG- -5' |
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17480 | 5' | -52.8 | NC_004456.1 | + | 20486 | 1.12 | 0.000707 |
Target: 5'- aAGCAUCCAGCCUUCAACGCCUUGACCu -3' miRNA: 3'- -UCGUAGGUCGGAAGUUGCGGAACUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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