Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17481 | 3' | -52.4 | NC_004456.1 | + | 18751 | 0.66 | 0.771138 |
Target: 5'- uGGCAGCGuuucaUCAGgaauaUCACUGGCCAgcaUAUCc -3' miRNA: 3'- -CUGUCGU-----AGUCg----AGUGGUCGGU---AUAG- -5' |
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17481 | 3' | -52.4 | NC_004456.1 | + | 32807 | 0.66 | 0.749379 |
Target: 5'- aGC-GCAUCAGCUCAUCaaAGuCCAUGc- -3' miRNA: 3'- cUGuCGUAGUCGAGUGG--UC-GGUAUag -5' |
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17481 | 3' | -52.4 | NC_004456.1 | + | 42906 | 0.66 | 0.738291 |
Target: 5'- cAUGGUAUgCAGUUCACCggacauuacagGGCCAUAUUc -3' miRNA: 3'- cUGUCGUA-GUCGAGUGG-----------UCGGUAUAG- -5' |
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17481 | 3' | -52.4 | NC_004456.1 | + | 35718 | 0.66 | 0.720305 |
Target: 5'- -cCGGCAUCgauaccagccuuggcGGCUaGCCAGCCA-GUCa -3' miRNA: 3'- cuGUCGUAG---------------UCGAgUGGUCGGUaUAG- -5' |
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17481 | 3' | -52.4 | NC_004456.1 | + | 30925 | 0.67 | 0.692869 |
Target: 5'- cGCAGCuguAUCGgcuacGCUCACCAGCUG-GUCa -3' miRNA: 3'- cUGUCG---UAGU-----CGAGUGGUCGGUaUAG- -5' |
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17481 | 3' | -52.4 | NC_004456.1 | + | 20199 | 0.67 | 0.681315 |
Target: 5'- gGGCAGCAUCGGUguaaUUugUGGCCGUc-- -3' miRNA: 3'- -CUGUCGUAGUCG----AGugGUCGGUAuag -5' |
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17481 | 3' | -52.4 | NC_004456.1 | + | 33221 | 0.67 | 0.669707 |
Target: 5'- gGGCAGC-UCGGC-CACCuGCCuu-UCu -3' miRNA: 3'- -CUGUCGuAGUCGaGUGGuCGGuauAG- -5' |
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17481 | 3' | -52.4 | NC_004456.1 | + | 19289 | 0.68 | 0.598494 |
Target: 5'- aGCAGguUCAGCaccacaugaucauUCACCAGUUcUGUCg -3' miRNA: 3'- cUGUCguAGUCG-------------AGUGGUCGGuAUAG- -5' |
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17481 | 3' | -52.4 | NC_004456.1 | + | 38900 | 0.7 | 0.475515 |
Target: 5'- -cCAGCGccCAGCU-AUCAGCCGUAUCu -3' miRNA: 3'- cuGUCGUa-GUCGAgUGGUCGGUAUAG- -5' |
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17481 | 3' | -52.4 | NC_004456.1 | + | 23988 | 0.71 | 0.443811 |
Target: 5'- aACGGCuuccacucUCGGUcuUCACCAGCCcgGUCa -3' miRNA: 3'- cUGUCGu-------AGUCG--AGUGGUCGGuaUAG- -5' |
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17481 | 3' | -52.4 | NC_004456.1 | + | 2052 | 0.74 | 0.289476 |
Target: 5'- aACAGCAUCAGC-CACU-GCCAcAUCg -3' miRNA: 3'- cUGUCGUAGUCGaGUGGuCGGUaUAG- -5' |
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17481 | 3' | -52.4 | NC_004456.1 | + | 20838 | 1.1 | 0.000896 |
Target: 5'- cGACAGCAUCAGCUCACCAGCCAUAUCu -3' miRNA: 3'- -CUGUCGUAGUCGAGUGGUCGGUAUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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