miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17481 5' -49 NC_004456.1 + 3207 0.66 0.876725
Target:  5'- --aGCAUggGCAcCAcGGAUGCCUg- -3'
miRNA:   3'- cacCGUGuuCGUaGUuUCUACGGAaa -5'
17481 5' -49 NC_004456.1 + 39141 0.67 0.867208
Target:  5'- cUGGCaaGCAGGCcgccgccAUCAAAGAggcauacaucaaUGCCUUUa -3'
miRNA:   3'- cACCG--UGUUCG-------UAGUUUCU------------ACGGAAA- -5'
17481 5' -49 NC_004456.1 + 18295 0.67 0.830679
Target:  5'- -cGGCACGAGCAggcCAGAG--GCCa-- -3'
miRNA:   3'- caCCGUGUUCGUa--GUUUCuaCGGaaa -5'
17481 5' -49 NC_004456.1 + 31173 0.68 0.789247
Target:  5'- -cGGCACAGGCcAUCA---AUGCCa-- -3'
miRNA:   3'- caCCGUGUUCG-UAGUuucUACGGaaa -5'
17481 5' -49 NC_004456.1 + 30586 0.71 0.613568
Target:  5'- gGUGGCAUcGGCAUCc--GGUGCCg-- -3'
miRNA:   3'- -CACCGUGuUCGUAGuuuCUACGGaaa -5'
17481 5' -49 NC_004456.1 + 21234 0.83 0.139962
Target:  5'- aUGGCAUGAGCAUCGAccacGGUGCCUUUg -3'
miRNA:   3'- cACCGUGUUCGUAGUUu---CUACGGAAA- -5'
17481 5' -49 NC_004456.1 + 20799 1.05 0.004176
Target:  5'- gGUGGCACAAGCAUCAAAGAUGCCUUUc -3'
miRNA:   3'- -CACCGUGUUCGUAGUUUCUACGGAAA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.