Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17481 | 5' | -49 | NC_004456.1 | + | 3207 | 0.66 | 0.876725 |
Target: 5'- --aGCAUggGCAcCAcGGAUGCCUg- -3' miRNA: 3'- cacCGUGuuCGUaGUuUCUACGGAaa -5' |
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17481 | 5' | -49 | NC_004456.1 | + | 39141 | 0.67 | 0.867208 |
Target: 5'- cUGGCaaGCAGGCcgccgccAUCAAAGAggcauacaucaaUGCCUUUa -3' miRNA: 3'- cACCG--UGUUCG-------UAGUUUCU------------ACGGAAA- -5' |
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17481 | 5' | -49 | NC_004456.1 | + | 18295 | 0.67 | 0.830679 |
Target: 5'- -cGGCACGAGCAggcCAGAG--GCCa-- -3' miRNA: 3'- caCCGUGUUCGUa--GUUUCuaCGGaaa -5' |
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17481 | 5' | -49 | NC_004456.1 | + | 31173 | 0.68 | 0.789247 |
Target: 5'- -cGGCACAGGCcAUCA---AUGCCa-- -3' miRNA: 3'- caCCGUGUUCG-UAGUuucUACGGaaa -5' |
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17481 | 5' | -49 | NC_004456.1 | + | 30586 | 0.71 | 0.613568 |
Target: 5'- gGUGGCAUcGGCAUCc--GGUGCCg-- -3' miRNA: 3'- -CACCGUGuUCGUAGuuuCUACGGaaa -5' |
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17481 | 5' | -49 | NC_004456.1 | + | 21234 | 0.83 | 0.139962 |
Target: 5'- aUGGCAUGAGCAUCGAccacGGUGCCUUUg -3' miRNA: 3'- cACCGUGUUCGUAGUUu---CUACGGAAA- -5' |
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17481 | 5' | -49 | NC_004456.1 | + | 20799 | 1.05 | 0.004176 |
Target: 5'- gGUGGCACAAGCAUCAAAGAUGCCUUUc -3' miRNA: 3'- -CACCGUGUUCGUAGUUUCUACGGAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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