miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17482 3' -56.6 NC_004456.1 + 32186 0.66 0.535505
Target:  5'- cCUGacuacGCCGCCAGgcagcaaccugcUGGCCGGUGgCUUCa -3'
miRNA:   3'- -GACcu---UGGCGGUC------------ACUGGCCGU-GAAG- -5'
17482 3' -56.6 NC_004456.1 + 19874 0.67 0.514053
Target:  5'- aCUGGccuguguUUGCCAG-GGCCuGCACUUCu -3'
miRNA:   3'- -GACCuu-----GGCGGUCaCUGGcCGUGAAG- -5'
17482 3' -56.6 NC_004456.1 + 21310 0.67 0.492961
Target:  5'- -----cCCGCCAGUGAgCGGguUUUCu -3'
miRNA:   3'- gaccuuGGCGGUCACUgGCCguGAAG- -5'
17482 3' -56.6 NC_004456.1 + 42258 0.69 0.376184
Target:  5'- uUGGuauGCCGUCAGUGACUuccuGGCgaGCUUUu -3'
miRNA:   3'- gACCu--UGGCGGUCACUGG----CCG--UGAAG- -5'
17482 3' -56.6 NC_004456.1 + 21085 0.71 0.291952
Target:  5'- gUGGugaacGCUGCCGGUGACCugauuguuccaggcGGCACUg- -3'
miRNA:   3'- gACCu----UGGCGGUCACUGG--------------CCGUGAag -5'
17482 3' -56.6 NC_004456.1 + 17165 0.71 0.272461
Target:  5'- uUGGaAACCGCUguaUGACCGGCGCg-- -3'
miRNA:   3'- gACC-UUGGCGGuc-ACUGGCCGUGaag -5'
17482 3' -56.6 NC_004456.1 + 24589 1.1 0.000453
Target:  5'- aCUGGAACCGCCAGUGACCGGCACUUCu -3'
miRNA:   3'- -GACCUUGGCGGUCACUGGCCGUGAAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.