Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17485 | 3' | -53.9 | NC_004456.1 | + | 23162 | 0.66 | 0.679898 |
Target: 5'- gAGCGGUacuaucagCGggCGCAAGGGCGGUUUACu -3' miRNA: 3'- -UCGUCG--------GUaaGCGUUUCUGUCGGGUG- -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 1237 | 0.66 | 0.656924 |
Target: 5'- uGGaauGCUAUUCGaCAuAGAUGGCCCGg -3' miRNA: 3'- -UCgu-CGGUAAGC-GUuUCUGUCGGGUg -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 26513 | 0.67 | 0.622304 |
Target: 5'- cAGCAGCCAUguuuUUGUuuguGGugGGCUgGCu -3' miRNA: 3'- -UCGUCGGUA----AGCGuu--UCugUCGGgUG- -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 14732 | 0.67 | 0.603855 |
Target: 5'- cAGCGGCCAaccUuaucgaccaaaccagUCGCcuGGGCaAGCCCAa -3' miRNA: 3'- -UCGUCGGU---A---------------AGCGuuUCUG-UCGGGUg -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 8396 | 0.67 | 0.599252 |
Target: 5'- aGGCAGCC---UGgGAAGACAGgCCGg -3' miRNA: 3'- -UCGUCGGuaaGCgUUUCUGUCgGGUg -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 16367 | 0.67 | 0.572899 |
Target: 5'- uAGUAGCCGauaGCAAGGuCaacaauuuugguguAGCCCACa -3' miRNA: 3'- -UCGUCGGUaagCGUUUCuG--------------UCGGGUG- -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 31301 | 0.68 | 0.564929 |
Target: 5'- cGGaCAGCCugGUUCGCAAccaccuguGGGCGGUugaCCGCa -3' miRNA: 3'- -UC-GUCGG--UAAGCGUU--------UCUGUCG---GGUG- -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 33485 | 0.68 | 0.554726 |
Target: 5'- cGGCAgGCCAaaaagcuggagagaaUCGCAGAGAUagaAGCCCGu -3' miRNA: 3'- -UCGU-CGGUa--------------AGCGUUUCUG---UCGGGUg -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 18283 | 0.68 | 0.553595 |
Target: 5'- uGGCGGCCAccgUUgGCAggacaucgGAGACuauGGCCCGg -3' miRNA: 3'- -UCGUCGGU---AAgCGU--------UUCUG---UCGGGUg -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 20609 | 0.68 | 0.54233 |
Target: 5'- aAGCGGUgAaaagCGCAAAGGCcgaAGCCaCACa -3' miRNA: 3'- -UCGUCGgUaa--GCGUUUCUG---UCGG-GUG- -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 8648 | 0.68 | 0.520039 |
Target: 5'- uAGgGGCCGguUUUGCAaaccAAGACGGCaCCAUu -3' miRNA: 3'- -UCgUCGGU--AAGCGU----UUCUGUCG-GGUG- -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 40237 | 0.68 | 0.520039 |
Target: 5'- aGGCuGCUuc-CGCAAucaGGGCAGCCgCACu -3' miRNA: 3'- -UCGuCGGuaaGCGUU---UCUGUCGG-GUG- -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 8791 | 0.69 | 0.48731 |
Target: 5'- cAGCAGCCAca-GCAAGGugguuaucaGCAGCCuCAg -3' miRNA: 3'- -UCGUCGGUaagCGUUUC---------UGUCGG-GUg -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 3413 | 0.69 | 0.480879 |
Target: 5'- cAGgAGCCAUUUGCGGAGuGCAuuugcuucuuugcuuGUCCACu -3' miRNA: 3'- -UCgUCGGUAAGCGUUUC-UGU---------------CGGGUG- -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 39549 | 0.69 | 0.476616 |
Target: 5'- cGUAGCCAUaccuguuaUgGCGGgauGACAGCCCGg -3' miRNA: 3'- uCGUCGGUA--------AgCGUUu--CUGUCGGGUg -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 15927 | 0.69 | 0.455586 |
Target: 5'- uAGCAGCCuggUCGCA---GCuGCCCGu -3' miRNA: 3'- -UCGUCGGua-AGCGUuucUGuCGGGUg -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 27729 | 0.7 | 0.415093 |
Target: 5'- -uUAGCCAgagaccagagUGCAAuGGCGGCCCACu -3' miRNA: 3'- ucGUCGGUaa--------GCGUUuCUGUCGGGUG- -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 19827 | 0.7 | 0.415093 |
Target: 5'- aAGCGGUCGUgcaacgccaUCGCAAuGGCcgacaaaucacuGGCCCGCa -3' miRNA: 3'- -UCGUCGGUA---------AGCGUUuCUG------------UCGGGUG- -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 20821 | 0.71 | 0.386213 |
Target: 5'- gAGCAGCUGUUCGCuAAGAUGGCg--- -3' miRNA: 3'- -UCGUCGGUAAGCGuUUCUGUCGggug -5' |
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17485 | 3' | -53.9 | NC_004456.1 | + | 32767 | 0.71 | 0.373197 |
Target: 5'- cGGCGGCCAauggaagaccgcCGCAAuauCGGCCCACg -3' miRNA: 3'- -UCGUCGGUaa----------GCGUUucuGUCGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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