miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17486 3' -63.7 NC_004456.1 + 4181 0.66 0.240879
Target:  5'- cGAGCCcugGCCaGCUGCUGGUGGucuuguucugcugUGCCAGu -3'
miRNA:   3'- -CUCGG---CGGgCGGCGGCUACU-------------GCGGUC- -5'
17486 3' -63.7 NC_004456.1 + 13489 0.71 0.119008
Target:  5'- aAGCCG-CUGCCGuuGAUG-CGCCAc -3'
miRNA:   3'- cUCGGCgGGCGGCggCUACuGCGGUc -5'
17486 3' -63.7 NC_004456.1 + 15381 0.69 0.163631
Target:  5'- cAGCCcgcaucguuacuaGCCCaGUgGCCGAUGugGCuCAGg -3'
miRNA:   3'- cUCGG-------------CGGG-CGgCGGCUACugCG-GUC- -5'
17486 3' -63.7 NC_004456.1 + 23487 0.67 0.235491
Target:  5'- gGAGCUGUgggaauuuuCCGCCGCUG-UGGC-CCAGc -3'
miRNA:   3'- -CUCGGCG---------GGCGGCGGCuACUGcGGUC- -5'
17486 3' -63.7 NC_004456.1 + 27592 1.08 0.000135
Target:  5'- aGAGCCGCCCGCCGCCGAUGACGCCAGa -3'
miRNA:   3'- -CUCGGCGGGCGGCGGCUACUGCGGUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.