miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17488 5' -52.6 NC_004456.1 + 5614 0.66 0.774162
Target:  5'- gCCAGaCGGAGauuuuaGGCGAGGcaguuuuuugaaUUGGGCGu -3'
miRNA:   3'- aGGUCaGCCUCg-----UCGUUCC------------AAUUCGU- -5'
17488 5' -52.6 NC_004456.1 + 7875 0.66 0.760288
Target:  5'- gUUCAGUCGGAuggugcggcuauguGC-GCAGGGUUGugGGUg -3'
miRNA:   3'- -AGGUCAGCCU--------------CGuCGUUCCAAU--UCGu -5'
17488 5' -52.6 NC_004456.1 + 35223 0.66 0.741779
Target:  5'- -gCGGUCGGuGUGGCu-GGUgcGGCAu -3'
miRNA:   3'- agGUCAGCCuCGUCGuuCCAauUCGU- -5'
17488 5' -52.6 NC_004456.1 + 2478 0.66 0.730723
Target:  5'- cCCAGaaGGAGCuGGCAgaucAGGUggguaUGAGCGa -3'
miRNA:   3'- aGGUCagCCUCG-UCGU----UCCA-----AUUCGU- -5'
17488 5' -52.6 NC_004456.1 + 8315 0.67 0.704899
Target:  5'- gUCAGUUGGAGCgugaguauagccuuGGCGGuGGUcauUAAGCGa -3'
miRNA:   3'- aGGUCAGCCUCG--------------UCGUU-CCA---AUUCGU- -5'
17488 5' -52.6 NC_004456.1 + 34225 0.96 0.010602
Target:  5'- cUCCAGUC-GAGCAGCAAGGUUAAGCAc -3'
miRNA:   3'- -AGGUCAGcCUCGUCGUUCCAAUUCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.