Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17488 | 5' | -52.6 | NC_004456.1 | + | 5614 | 0.66 | 0.774162 |
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Target: 5'- gCCAGaCGGAGauuuuaGGCGAGGcaguuuuuugaaUUGGGCGu -3' miRNA: 3'- aGGUCaGCCUCg-----UCGUUCC------------AAUUCGU- -5' |
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| 17488 | 5' | -52.6 | NC_004456.1 | + | 7875 | 0.66 | 0.760288 |
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Target: 5'- gUUCAGUCGGAuggugcggcuauguGC-GCAGGGUUGugGGUg -3' miRNA: 3'- -AGGUCAGCCU--------------CGuCGUUCCAAU--UCGu -5' |
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| 17488 | 5' | -52.6 | NC_004456.1 | + | 35223 | 0.66 | 0.741779 |
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Target: 5'- -gCGGUCGGuGUGGCu-GGUgcGGCAu -3' miRNA: 3'- agGUCAGCCuCGUCGuuCCAauUCGU- -5' |
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| 17488 | 5' | -52.6 | NC_004456.1 | + | 2478 | 0.66 | 0.730723 |
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Target: 5'- cCCAGaaGGAGCuGGCAgaucAGGUggguaUGAGCGa -3' miRNA: 3'- aGGUCagCCUCG-UCGU----UCCA-----AUUCGU- -5' |
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| 17488 | 5' | -52.6 | NC_004456.1 | + | 8315 | 0.67 | 0.704899 |
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Target: 5'- gUCAGUUGGAGCgugaguauagccuuGGCGGuGGUcauUAAGCGa -3' miRNA: 3'- aGGUCAGCCUCG--------------UCGUU-CCA---AUUCGU- -5' |
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| 17488 | 5' | -52.6 | NC_004456.1 | + | 34225 | 0.96 | 0.010602 |
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Target: 5'- cUCCAGUC-GAGCAGCAAGGUUAAGCAc -3' miRNA: 3'- -AGGUCAGcCUCGUCGUUCCAAUUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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