Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17492 | 5' | -52.7 | NC_004456.1 | + | 7849 | 0.66 | 0.754351 |
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Target: 5'- -cGGcgGCGGCCAUGaugGCCAGuuccaguuguAACcGCu -3' miRNA: 3'- cuCCa-CGCCGGUACaa-CGGUU----------UUG-CG- -5' |
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| 17492 | 5' | -52.7 | NC_004456.1 | + | 36877 | 0.66 | 0.73251 |
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Target: 5'- aGGGGUGCGGaCCuuc--GCCAGGA-GCu -3' miRNA: 3'- -CUCCACGCC-GGuacaaCGGUUUUgCG- -5' |
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| 17492 | 5' | -52.7 | NC_004456.1 | + | 1321 | 0.67 | 0.710229 |
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Target: 5'- -cGGUGUGGUugaUAUGg-GCCAAAACGa -3' miRNA: 3'- cuCCACGCCG---GUACaaCGGUUUUGCg -5' |
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| 17492 | 5' | -52.7 | NC_004456.1 | + | 18274 | 0.67 | 0.710229 |
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Target: 5'- aGGGGccagugGCGGCCAccGUUGgCAGGACa- -3' miRNA: 3'- -CUCCa-----CGCCGGUa-CAACgGUUUUGcg -5' |
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| 17492 | 5' | -52.7 | NC_004456.1 | + | 15323 | 0.68 | 0.664763 |
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Target: 5'- --aGUGUGGCCgGUGaUGCCcu-GCGCg -3' miRNA: 3'- cucCACGCCGG-UACaACGGuuuUGCG- -5' |
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| 17492 | 5' | -52.7 | NC_004456.1 | + | 7886 | 0.68 | 0.653282 |
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Target: 5'- -uGGUGCGGCUAUGU-GCgCAGggUuGUg -3' miRNA: 3'- cuCCACGCCGGUACAaCG-GUUuuG-CG- -5' |
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| 17492 | 5' | -52.7 | NC_004456.1 | + | 14319 | 0.68 | 0.640628 |
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Target: 5'- -cGGUGUuaacucagguacaGGCCGUGcgGCCAAguuccugcAACGCc -3' miRNA: 3'- cuCCACG-------------CCGGUACaaCGGUU--------UUGCG- -5' |
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| 17492 | 5' | -52.7 | NC_004456.1 | + | 36158 | 1.12 | 0.000768 |
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Target: 5'- uGAGGUGCGGCCAUGUUGCCAAAACGCu -3' miRNA: 3'- -CUCCACGCCGGUACAACGGUUUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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