Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17495 | 3' | -56.3 | NC_004456.1 | + | 7787 | 0.66 | 0.546583 |
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Target: 5'- -uGGCCAUCAUgGCC-GCCGCCguaGAa- -3' miRNA: 3'- uuUCGGUAGUG-CGGuCGGUGGa--CUgu -5' |
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| 17495 | 3' | -56.3 | NC_004456.1 | + | 29978 | 0.66 | 0.524534 |
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Target: 5'- -uGGCCAUgcgggcCACGCCAGC-ACCU-ACAa -3' miRNA: 3'- uuUCGGUA------GUGCGGUCGgUGGAcUGU- -5' |
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| 17495 | 3' | -56.3 | NC_004456.1 | + | 32875 | 0.66 | 0.513635 |
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Target: 5'- -uGGCCGUCAaGCUccaAGCCAUC-GACAa -3' miRNA: 3'- uuUCGGUAGUgCGG---UCGGUGGaCUGU- -5' |
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| 17495 | 3' | -56.3 | NC_004456.1 | + | 22677 | 0.67 | 0.460675 |
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Target: 5'- -uAGCCgGUCAC-CguGaCCACCUGGCAc -3' miRNA: 3'- uuUCGG-UAGUGcGguC-GGUGGACUGU- -5' |
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| 17495 | 3' | -56.3 | NC_004456.1 | + | 32271 | 0.68 | 0.430318 |
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Target: 5'- uGAAGCCAcCG-GCCAGCagguugcuGCCUGGCGg -3' miRNA: 3'- -UUUCGGUaGUgCGGUCGg-------UGGACUGU- -5' |
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| 17495 | 3' | -56.3 | NC_004456.1 | + | 2783 | 0.69 | 0.364316 |
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Target: 5'- --uGCCGUUcacuCGCC-GCCACuCUGACAu -3' miRNA: 3'- uuuCGGUAGu---GCGGuCGGUG-GACUGU- -5' |
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| 17495 | 3' | -56.3 | NC_004456.1 | + | 17997 | 0.69 | 0.346789 |
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Target: 5'- cGAGCCAUCAaagaGCUGGCUAUC-GGCAa -3' miRNA: 3'- uUUCGGUAGUg---CGGUCGGUGGaCUGU- -5' |
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| 17495 | 3' | -56.3 | NC_004456.1 | + | 31523 | 0.69 | 0.34165 |
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Target: 5'- cAGAGCaCAUCACGUuccgCAGCCACaugguuaacggcuauCUGACGg -3' miRNA: 3'- -UUUCG-GUAGUGCG----GUCGGUG---------------GACUGU- -5' |
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| 17495 | 3' | -56.3 | NC_004456.1 | + | 412 | 0.7 | 0.316785 |
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Target: 5'- -uGGCUgauuuguggauugCugGCCAGCuCACCUGACAc -3' miRNA: 3'- uuUCGGua-----------GugCGGUCG-GUGGACUGU- -5' |
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| 17495 | 3' | -56.3 | NC_004456.1 | + | 37528 | 1.06 | 0.00068 |
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Target: 5'- uAAAGCCAUCACGCCAGCCACCUGACAa -3' miRNA: 3'- -UUUCGGUAGUGCGGUCGGUGGACUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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