Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17496 | 5' | -57.6 | NC_004456.1 | + | 35536 | 0.67 | 0.444994 |
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Target: 5'- uGGCGGGUCUUUUca--GCCuCGGCCa -3' miRNA: 3'- gCCGCUCAGAAGGaaacCGG-GUCGGa -5' |
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| 17496 | 5' | -57.6 | NC_004456.1 | + | 27536 | 0.67 | 0.43519 |
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Target: 5'- gCGGCGGGcggcUCUgCCguuaucggUGGCCCucAGCCg -3' miRNA: 3'- -GCCGCUC----AGAaGGaa------ACCGGG--UCGGa -5' |
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| 17496 | 5' | -57.6 | NC_004456.1 | + | 41209 | 0.68 | 0.388122 |
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Target: 5'- uGGCGAGUCcgCC--UGGCCacccGCCg -3' miRNA: 3'- gCCGCUCAGaaGGaaACCGGgu--CGGa -5' |
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| 17496 | 5' | -57.6 | NC_004456.1 | + | 22336 | 0.69 | 0.352956 |
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Target: 5'- uGGuUGAGUUggaUgCUgcGGCCCAGCCUg -3' miRNA: 3'- gCC-GCUCAGa--AgGAaaCCGGGUCGGA- -5' |
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| 17496 | 5' | -57.6 | NC_004456.1 | + | 32702 | 0.73 | 0.19017 |
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Target: 5'- gCGGCG-GUCUUCCaUUGGCC--GCCg -3' miRNA: 3'- -GCCGCuCAGAAGGaAACCGGguCGGa -5' |
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| 17496 | 5' | -57.6 | NC_004456.1 | + | 38483 | 1.07 | 0.000568 |
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Target: 5'- uCGGCGAGUCUUCCUUUGGCCCAGCCUu -3' miRNA: 3'- -GCCGCUCAGAAGGAAACCGGGUCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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