Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17498 | 5' | -54.6 | NC_004456.1 | + | 17616 | 0.67 | 0.613141 |
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Target: 5'- cGUUGCuGCGGCUCGguggCUUAaucGCCg -3' miRNA: 3'- -CGACGcCGUCGAGCaga-GAAUga-CGG- -5' |
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| 17498 | 5' | -54.6 | NC_004456.1 | + | 22897 | 0.67 | 0.579413 |
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Target: 5'- aCUGUGcGUucaAGCacgCGUCUCUUAC-GCCg -3' miRNA: 3'- cGACGC-CG---UCGa--GCAGAGAAUGaCGG- -5' |
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| 17498 | 5' | -54.6 | NC_004456.1 | + | 37358 | 0.68 | 0.546097 |
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Target: 5'- gGCUGCGGCcucugAGUUgGcCUCUUugcaGCgugGCCg -3' miRNA: 3'- -CGACGCCG-----UCGAgCaGAGAA----UGa--CGG- -5' |
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| 17498 | 5' | -54.6 | NC_004456.1 | + | 40954 | 0.68 | 0.546097 |
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Target: 5'- --gGCGGCGGCUUcUUUaCUUACcGCCu -3' miRNA: 3'- cgaCGCCGUCGAGcAGA-GAAUGaCGG- -5' |
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| 17498 | 5' | -54.6 | NC_004456.1 | + | 36033 | 0.7 | 0.431731 |
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Target: 5'- gGCUGCccguuccgugGGCAGCguuugguuugcuaaGUCUCUUGCcaggGCCu -3' miRNA: 3'- -CGACG----------CCGUCGag------------CAGAGAAUGa---CGG- -5' |
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| 17498 | 5' | -54.6 | NC_004456.1 | + | 33224 | 0.72 | 0.347773 |
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Target: 5'- cCUG-GGCAGCUCGgccaccugccUUUC-UGCUGCCg -3' miRNA: 3'- cGACgCCGUCGAGC----------AGAGaAUGACGG- -5' |
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| 17498 | 5' | -54.6 | NC_004456.1 | + | 2656 | 1.14 | 0.000377 |
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Target: 5'- gGCUGCGGCAGCUCGUCUCUUACUGCCu -3' miRNA: 3'- -CGACGCCGUCGAGCAGAGAAUGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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