miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17499 3' -60.9 NC_004456.1 + 38495 0.66 0.342678
Target:  5'- uCGCCguuugggucgGCGAGUCUuccuuUGGcCCAGCcuugUGCUGGg -3'
miRNA:   3'- -GUGG----------UGCUCGGG-----ACC-GGUCG----ACGACC- -5'
17499 3' -60.9 NC_004456.1 + 35812 0.66 0.334594
Target:  5'- cCACCGCcuGCCaugacuggCUGGCUAGCcgccaagGCUGGu -3'
miRNA:   3'- -GUGGUGcuCGG--------GACCGGUCGa------CGACC- -5'
17499 3' -60.9 NC_004456.1 + 30355 0.67 0.289041
Target:  5'- uGCCuaauCGaAGCCCUGGCCuG-UGCcGGg -3'
miRNA:   3'- gUGGu---GC-UCGGGACCGGuCgACGaCC- -5'
17499 3' -60.9 NC_004456.1 + 20138 0.69 0.230058
Target:  5'- cCGCUGCuuauGCCUUGGCCuGCUGUUGu -3'
miRNA:   3'- -GUGGUGcu--CGGGACCGGuCGACGACc -5'
17499 3' -60.9 NC_004456.1 + 25454 0.69 0.224171
Target:  5'- gUugCACGGGCUCgGGCUGGUUGCa-- -3'
miRNA:   3'- -GugGUGCUCGGGaCCGGUCGACGacc -5'
17499 3' -60.9 NC_004456.1 + 4175 1.09 0.000176
Target:  5'- aCACCACGAGCCCUGGCCAGCUGCUGGu -3'
miRNA:   3'- -GUGGUGCUCGGGACCGGUCGACGACC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.