Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 175 | 5' | -50.6 | AC_000007.1 | + | 14319 | 0.66 | 0.83284 |
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Target: 5'- ----cGGUAGgCcGCAGGUACCGCGGa -3' miRNA: 3'- cgucuCCAUUgGaUGUUCGUGGUGCU- -5' |
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| 175 | 5' | -50.6 | AC_000007.1 | + | 26843 | 0.67 | 0.803127 |
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Target: 5'- aGCAGgAGGaggAGCgCUGCGucuGGCGCCcaACGAa -3' miRNA: 3'- -CGUC-UCCa--UUG-GAUGU---UCGUGG--UGCU- -5' |
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| 175 | 5' | -50.6 | AC_000007.1 | + | 10746 | 0.67 | 0.803127 |
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Target: 5'- cGCGGGcGGUAACC-GCAuGgAUCACGGc -3' miRNA: 3'- -CGUCU-CCAUUGGaUGUuCgUGGUGCU- -5' |
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| 175 | 5' | -50.6 | AC_000007.1 | + | 24325 | 0.67 | 0.760714 |
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Target: 5'- gGCGGGGG-GACCaa-AGGCAUgGCGAc -3' miRNA: 3'- -CGUCUCCaUUGGaugUUCGUGgUGCU- -5' |
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| 175 | 5' | -50.6 | AC_000007.1 | + | 17706 | 0.67 | 0.760714 |
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Target: 5'- aGguGAGGgccauAUCUGCAAGaACCACa- -3' miRNA: 3'- -CguCUCCau---UGGAUGUUCgUGGUGcu -5' |
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| 175 | 5' | -50.6 | AC_000007.1 | + | 17348 | 0.68 | 0.715896 |
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Target: 5'- cGUAGAGGUuuuggacgcGGCCgcaGCGGccgccuGCACCGCGGc -3' miRNA: 3'- -CGUCUCCA---------UUGGa--UGUU------CGUGGUGCU- -5' |
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| 175 | 5' | -50.6 | AC_000007.1 | + | 8675 | 0.68 | 0.70442 |
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Target: 5'- cGCGGAGGUuGCUgGCGAaCGCgACGAc -3' miRNA: 3'- -CGUCUCCAuUGGaUGUUcGUGgUGCU- -5' |
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| 175 | 5' | -50.6 | AC_000007.1 | + | 23327 | 1.12 | 0.001074 |
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Target: 5'- uGCAGAGGUAACCUACAAGCACCACGAg -3' miRNA: 3'- -CGUCUCCAUUGGAUGUUCGUGGUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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