Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17501 | 5' | -48.1 | NC_004456.1 | + | 4012 | 0.66 | 0.959687 |
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Target: 5'- -uACCGCCAGCGAUUcaucagccaggcaaaAGCAGaAGGc-- -3' miRNA: 3'- ccUGGUGGUCGUUAA---------------UUGUC-UCCuua -5' |
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| 17501 | 5' | -48.1 | NC_004456.1 | + | 3231 | 0.66 | 0.93821 |
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Target: 5'- ---gCACCuGCAGgaAGCAGAGGAc- -3' miRNA: 3'- ccugGUGGuCGUUaaUUGUCUCCUua -5' |
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| 17501 | 5' | -48.1 | NC_004456.1 | + | 37494 | 0.67 | 0.932469 |
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Target: 5'- cGGCUuUCAGCAAUgacCAGAGGAu- -3' miRNA: 3'- cCUGGuGGUCGUUAauuGUCUCCUua -5' |
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| 17501 | 5' | -48.1 | NC_004456.1 | + | 9647 | 0.69 | 0.857526 |
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Target: 5'- uGACCGCCugguugagcgAGCAAUgGAcCAGGGGGAc -3' miRNA: 3'- cCUGGUGG----------UCGUUAaUU-GUCUCCUUa -5' |
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| 17501 | 5' | -48.1 | NC_004456.1 | + | 17100 | 0.69 | 0.857526 |
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Target: 5'- uGACCACuUGGCAuUUGGCAGGucGGAGUa -3' miRNA: 3'- cCUGGUG-GUCGUuAAUUGUCU--CCUUA- -5' |
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| 17501 | 5' | -48.1 | NC_004456.1 | + | 35485 | 0.7 | 0.788347 |
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Target: 5'- aGAcCCGCCAGCAA---GCGGAGGc-- -3' miRNA: 3'- cCU-GGUGGUCGUUaauUGUCUCCuua -5' |
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| 17501 | 5' | -48.1 | NC_004456.1 | + | 4250 | 0.7 | 0.788347 |
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Target: 5'- aGACCACCAGCAGcUGGCc-AGGGc- -3' miRNA: 3'- cCUGGUGGUCGUUaAUUGucUCCUua -5' |
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| 17501 | 5' | -48.1 | NC_004456.1 | + | 14022 | 0.72 | 0.698177 |
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Target: 5'- uGAuCCGCCAGCAcuauGCGGAGGGc- -3' miRNA: 3'- cCU-GGUGGUCGUuaauUGUCUCCUua -5' |
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| 17501 | 5' | -48.1 | NC_004456.1 | + | 30627 | 0.72 | 0.662721 |
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Target: 5'- cGGCUACCGGCGAUcaguaccggcgUAuuCAGAGGAAa -3' miRNA: 3'- cCUGGUGGUCGUUA-----------AUu-GUCUCCUUa -5' |
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| 17501 | 5' | -48.1 | NC_004456.1 | + | 13295 | 1.1 | 0.003162 |
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Target: 5'- aGGACCACCAGCAAUUAACAGAGGAAUa -3' miRNA: 3'- -CCUGGUGGUCGUUAAUUGUCUCCUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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