Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17502 | 5' | -59.8 | NC_004456.1 | + | 26227 | 0.66 | 0.397911 |
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Target: 5'- uGGUGCCGGGCUC-CUGgcuUCCGu-- -3' miRNA: 3'- gUCGCGGCCCGGGcGGCau-AGGUuuu -5' |
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| 17502 | 5' | -59.8 | NC_004456.1 | + | 38900 | 0.66 | 0.35388 |
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Target: 5'- cCAGCGCCcaGCUaucaGCCGUAUCUAu-- -3' miRNA: 3'- -GUCGCGGccCGGg---CGGCAUAGGUuuu -5' |
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| 17502 | 5' | -59.8 | NC_004456.1 | + | 28054 | 0.67 | 0.345497 |
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Target: 5'- aAGCGCaaCGGGCCUGCCGaugaCGAu- -3' miRNA: 3'- gUCGCG--GCCCGGGCGGCauagGUUuu -5' |
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| 17502 | 5' | -59.8 | NC_004456.1 | + | 36109 | 0.68 | 0.267531 |
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Target: 5'- aCAGCucCCGGGUgaaugccugguaagCUGCCGUAUCCAc-- -3' miRNA: 3'- -GUCGc-GGCCCG--------------GGCGGCAUAGGUuuu -5' |
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| 17502 | 5' | -59.8 | NC_004456.1 | + | 28272 | 0.69 | 0.236958 |
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Target: 5'- gAGCGCCGGGCUgggcgCGCUcUAUCUGAGc -3' miRNA: 3'- gUCGCGGCCCGG-----GCGGcAUAGGUUUu -5' |
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| 17502 | 5' | -59.8 | NC_004456.1 | + | 15849 | 1.05 | 0.000406 |
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Target: 5'- aCAGCGCCGGGCCCGCCGUAUCCAAAAc -3' miRNA: 3'- -GUCGCGGCCCGGGCGGCAUAGGUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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