Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17503 | 3' | -48.5 | NC_004456.1 | + | 30646 | 0.66 | 0.951916 |
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Target: 5'- uGACCAGacuGGCGCagugguggugguGAuucgcgugGAUGAGGgCGUGGa -3' miRNA: 3'- gCUGGUU---UCGUG------------CU--------UUACUCC-GUACC- -5' |
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| 17503 | 3' | -48.5 | NC_004456.1 | + | 39733 | 0.67 | 0.936607 |
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Target: 5'- -aGCCAGAG-ACGGAG-GAGGCAUa- -3' miRNA: 3'- gcUGGUUUCgUGCUUUaCUCCGUAcc -5' |
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| 17503 | 3' | -48.5 | NC_004456.1 | + | 42575 | 0.67 | 0.918502 |
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Target: 5'- uGGCCGucAGCGUGAAgcuGUGAGGCuacguUGGu -3' miRNA: 3'- gCUGGUu-UCGUGCUU---UACUCCGu----ACC- -5' |
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| 17503 | 3' | -48.5 | NC_004456.1 | + | 32721 | 0.68 | 0.911842 |
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Target: 5'- -cGCCGGAGCA---AAUGGacGGCAUGGa -3' miRNA: 3'- gcUGGUUUCGUgcuUUACU--CCGUACC- -5' |
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| 17503 | 3' | -48.5 | NC_004456.1 | + | 41096 | 0.68 | 0.890018 |
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Target: 5'- uGACCAAAGCGaacugGAAGUGAucacCAUGGu -3' miRNA: 3'- gCUGGUUUCGUg----CUUUACUcc--GUACC- -5' |
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| 17503 | 3' | -48.5 | NC_004456.1 | + | 31680 | 0.69 | 0.865527 |
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Target: 5'- aGACCGAGGaguucaaGGcagGGGGCAUGGa -3' miRNA: 3'- gCUGGUUUCgug----CUuuaCUCCGUACC- -5' |
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| 17503 | 3' | -48.5 | NC_004456.1 | + | 14051 | 0.7 | 0.809396 |
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Target: 5'- uGACUAAAGCACuaGAAAUGaAGGCu--- -3' miRNA: 3'- gCUGGUUUCGUG--CUUUAC-UCCGuacc -5' |
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| 17503 | 3' | -48.5 | NC_004456.1 | + | 15691 | 0.73 | 0.654071 |
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Target: 5'- gCGnCCAAAGguCGAAAU--GGCAUGGa -3' miRNA: 3'- -GCuGGUUUCguGCUUUAcuCCGUACC- -5' |
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| 17503 | 3' | -48.5 | NC_004456.1 | + | 17778 | 1.13 | 0.00207 |
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Target: 5'- cCGACCAAAGCACGAAAUGAGGCAUGGg -3' miRNA: 3'- -GCUGGUUUCGUGCUUUACUCCGUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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