Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17506 | 3' | -52.7 | NC_004456.1 | + | 17372 | 0.66 | 0.756121 |
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Target: 5'- uCAGCUG-ACCCGGGCUC---UGGAUa -3' miRNA: 3'- -GUCGAUgUGGGUUCGGGuuuACCUGc -5' |
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| 17506 | 3' | -52.7 | NC_004456.1 | + | 25388 | 0.67 | 0.678145 |
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Target: 5'- -uGCaACcaGCCCGAGCCCGugcaacAUGGugGu -3' miRNA: 3'- guCGaUG--UGGGUUCGGGUu-----UACCugC- -5' |
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| 17506 | 3' | -52.7 | NC_004456.1 | + | 13624 | 0.69 | 0.609256 |
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Target: 5'- uGGCUgugGCACCUGAGCCCAuaaAcgGGuCa -3' miRNA: 3'- gUCGA---UGUGGGUUCGGGU---UuaCCuGc -5' |
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| 17506 | 3' | -52.7 | NC_004456.1 | + | 18698 | 0.69 | 0.605813 |
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Target: 5'- gUAGCUGCAgaggaaccgaaagcCCCucGCUCA-AUGGACGa -3' miRNA: 3'- -GUCGAUGU--------------GGGuuCGGGUuUACCUGC- -5' |
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| 17506 | 3' | -52.7 | NC_004456.1 | + | 28251 | 0.69 | 0.586347 |
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Target: 5'- gGGCUACuggaacaaugGCUCGAGCgCCGGGcUGGGCGc -3' miRNA: 3'- gUCGAUG----------UGGGUUCG-GGUUU-ACCUGC- -5' |
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| 17506 | 3' | -52.7 | NC_004456.1 | + | 25983 | 0.7 | 0.497089 |
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Target: 5'- uGGCUG-GCCUggGCCCugucuGUGGAUGa -3' miRNA: 3'- gUCGAUgUGGGuuCGGGuu---UACCUGC- -5' |
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| 17506 | 3' | -52.7 | NC_004456.1 | + | 18474 | 0.72 | 0.424321 |
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Target: 5'- aCGGCUGCGgCCA--CCCAAGUGGccACGg -3' miRNA: 3'- -GUCGAUGUgGGUucGGGUUUACC--UGC- -5' |
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| 17506 | 3' | -52.7 | NC_004456.1 | + | 4169 | 0.72 | 0.391345 |
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Target: 5'- uCAGUUACACCaCGAGCCCuggccagcugcUGGugGu -3' miRNA: 3'- -GUCGAUGUGG-GUUCGGGuuu--------ACCugC- -5' |
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| 17506 | 3' | -52.7 | NC_004456.1 | + | 20582 | 1.1 | 0.001094 |
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Target: 5'- cCAGCUACACCCAAGCCCAAAUGGACGa -3' miRNA: 3'- -GUCGAUGUGGGUUCGGGUUUACCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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