Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17506 | 5' | -53.8 | NC_004456.1 | + | 35464 | 0.68 | 0.575583 |
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Target: 5'- --aGCAAuuGGCCGAGGCUgaaaagacccGCcaGCAAGCg -3' miRNA: 3'- ucgCGUUu-CCGGCUUCGG----------UG--UGUUCG- -5' |
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| 17506 | 5' | -53.8 | NC_004456.1 | + | 21860 | 0.67 | 0.609744 |
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Target: 5'- aAGaUGCGAAcguGGCCGAGucauGCCGC-CAAGUg -3' miRNA: 3'- -UC-GCGUUU---CCGGCUU----CGGUGuGUUCG- -5' |
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| 17506 | 5' | -53.8 | NC_004456.1 | + | 15956 | 0.67 | 0.620041 |
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Target: 5'- aGGUGCucgccauGAAGGCa-AGGCCACGacCGAGCu -3' miRNA: 3'- -UCGCG-------UUUCCGgcUUCGGUGU--GUUCG- -5' |
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| 17506 | 5' | -53.8 | NC_004456.1 | + | 23178 | 0.67 | 0.621186 |
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Target: 5'- gGGCGCAAGGGCgGuuuacuGAGCUGCugcgcCAAGa -3' miRNA: 3'- -UCGCGUUUCCGgC------UUCGGUGu----GUUCg -5' |
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| 17506 | 5' | -53.8 | NC_004456.1 | + | 24477 | 0.67 | 0.655532 |
|
Target: 5'- aAGCcauuGCAGAGGCCGuggugGAGCauauCACcgGAGCg -3' miRNA: 3'- -UCG----CGUUUCCGGC-----UUCGgu--GUG--UUCG- -5' |
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| 17506 | 5' | -53.8 | NC_004456.1 | + | 1336 | 0.66 | 0.666949 |
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Target: 5'- gGGC-CAaaacGAGGCUGAcaAGUCAUACAacaAGCa -3' miRNA: 3'- -UCGcGU----UUCCGGCU--UCGGUGUGU---UCG- -5' |
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| 17506 | 5' | -53.8 | NC_004456.1 | + | 20619 | 1.13 | 0.000472 |
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Target: 5'- aAGCGCAAAGGCCGAAGCCACACAAGCg -3' miRNA: 3'- -UCGCGUUUCCGGCUUCGGUGUGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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