Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17508 | 5' | -54 | NC_004456.1 | + | 24899 | 0.66 | 0.712177 |
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Target: 5'- cGGCCuGCAGgUGG-GGUGAUAUGaCcGCa -3' miRNA: 3'- -CUGG-UGUUgACCgCCACUGUAC-GaCG- -5' |
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| 17508 | 5' | -54 | NC_004456.1 | + | 31819 | 0.66 | 0.678515 |
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Target: 5'- -uUCAUAGCUGGCcacGGUGAa--GUUGCa -3' miRNA: 3'- cuGGUGUUGACCG---CCACUguaCGACG- -5' |
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| 17508 | 5' | -54 | NC_004456.1 | + | 23696 | 0.67 | 0.620481 |
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Target: 5'- cGCCGCAagagauuuaccugGCguaugccccgGGCGGUGAUGUGCcaccgGCa -3' miRNA: 3'- cUGGUGU-------------UGa---------CCGCCACUGUACGa----CG- -5' |
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| 17508 | 5' | -54 | NC_004456.1 | + | 5599 | 0.68 | 0.553762 |
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Target: 5'- -gUCGgGACggcGGCGGUGAUuAUGUUGCu -3' miRNA: 3'- cuGGUgUUGa--CCGCCACUG-UACGACG- -5' |
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| 17508 | 5' | -54 | NC_004456.1 | + | 40812 | 0.7 | 0.47769 |
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Target: 5'- aGCCACuGCaGGCuGUGuuaguguCAUGCUGCg -3' miRNA: 3'- cUGGUGuUGaCCGcCACu------GUACGACG- -5' |
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| 17508 | 5' | -54 | NC_004456.1 | + | 29364 | 0.7 | 0.456892 |
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Target: 5'- -cCCACAAa-GGCGcUGACAggGCUGCa -3' miRNA: 3'- cuGGUGUUgaCCGCcACUGUa-CGACG- -5' |
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| 17508 | 5' | -54 | NC_004456.1 | + | 22730 | 1.13 | 0.000474 |
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Target: 5'- aGACCACAACUGGCGGUGACAUGCUGCa -3' miRNA: 3'- -CUGGUGUUGACCGCCACUGUACGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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