Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1751 | 5' | -50 | NC_001347.2 | + | 206183 | 0.66 | 0.999698 |
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Target: 5'- uCUUUCCCCgUCCaGGUAGguGGGGUAGGc -3' miRNA: 3'- -GGAAGGGG-GGG-CUAUUacUUCCAUUC- -5' |
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| 1751 | 5' | -50 | NC_001347.2 | + | 183303 | 0.66 | 0.99952 |
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Target: 5'- --gUCCUCcagggugcgugagCCCGGgcguuUGAUGGAGGUGAGg -3' miRNA: 3'- ggaAGGGG-------------GGGCU-----AUUACUUCCAUUC- -5' |
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| 1751 | 5' | -50 | NC_001347.2 | + | 153611 | 0.66 | 0.99942 |
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Target: 5'- gUUUCaCCUCCUGGacgUGGUGggGGUGcAGg -3' miRNA: 3'- gGAAG-GGGGGGCU---AUUACuuCCAU-UC- -5' |
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| 1751 | 5' | -50 | NC_001347.2 | + | 54201 | 0.7 | 0.984061 |
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Target: 5'- cCCUUCCCCUCCGugu-UGuAGGUu-- -3' miRNA: 3'- -GGAAGGGGGGGCuauuACuUCCAuuc -5' |
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| 1751 | 5' | -50 | NC_001347.2 | + | 136706 | 0.75 | 0.893054 |
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Target: 5'- ---cCCCCCCCGGUuccggaggagagGggGggGGUGAGg -3' miRNA: 3'- ggaaGGGGGGGCUA------------UuaCuuCCAUUC- -5' |
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| 1751 | 5' | -50 | NC_001347.2 | + | 61712 | 1.11 | 0.013588 |
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Target: 5'- gCCUUCCCCCCCGAUAAUGAAGGUAAGu -3' miRNA: 3'- -GGAAGGGGGGGCUAUUACUUCCAUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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