Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17510 | 3' | -50.3 | NC_004456.1 | + | 35612 | 0.66 | 0.898124 |
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Target: 5'- uGCCAGugccGCAGCCAUgCCAgcggcAGcGGAg- -3' miRNA: 3'- -CGGUCuu--UGUUGGUAgGGU-----UC-CCUac -5' |
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| 17510 | 3' | -50.3 | NC_004456.1 | + | 20816 | 0.66 | 0.890607 |
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Target: 5'- cCCAGGAGCAGCUGUUCgCuaagauGGcGGAUGa -3' miRNA: 3'- cGGUCUUUGUUGGUAGG-Gu-----UC-CCUAC- -5' |
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| 17510 | 3' | -50.3 | NC_004456.1 | + | 2707 | 0.66 | 0.890607 |
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Target: 5'- uGCC----GCAGCCAcaUCCCAuGGGGUu -3' miRNA: 3'- -CGGucuuUGUUGGU--AGGGUuCCCUAc -5' |
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| 17510 | 3' | -50.3 | NC_004456.1 | + | 38977 | 0.68 | 0.810631 |
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Target: 5'- cGCCAGAuaacAGCAAUCAUCCUugauGGAa- -3' miRNA: 3'- -CGGUCU----UUGUUGGUAGGGuuc-CCUac -5' |
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| 17510 | 3' | -50.3 | NC_004456.1 | + | 24730 | 0.73 | 0.507222 |
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Target: 5'- cGCCGGuAGACAcgGCUA-CCCAGcGGGGUGg -3' miRNA: 3'- -CGGUC-UUUGU--UGGUaGGGUU-CCCUAC- -5' |
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| 17510 | 3' | -50.3 | NC_004456.1 | + | 34753 | 0.76 | 0.356031 |
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Target: 5'- aGCCAGcuGACAGCCGUCCguAuGGGAUu -3' miRNA: 3'- -CGGUCu-UUGUUGGUAGGguU-CCCUAc -5' |
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| 17510 | 3' | -50.3 | NC_004456.1 | + | 28931 | 0.97 | 0.016725 |
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Target: 5'- aGCCAGAAACAA-CAUCCCAAGGGAUGc -3' miRNA: 3'- -CGGUCUUUGUUgGUAGGGUUCCCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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