Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17514 | 3' | -58.3 | NC_004456.1 | + | 27184 | 0.66 | 0.468806 |
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Target: 5'- ---aGCCGCaucUCAGGCGCUGGCUc -3' miRNA: 3'- gcacCGGUGcauAGUCCGUGGCCGAc -5' |
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| 17514 | 3' | -58.3 | NC_004456.1 | + | 36719 | 0.68 | 0.348701 |
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Target: 5'- -aUGGUCAUuucUAUCAGGCGCuCGGCa- -3' miRNA: 3'- gcACCGGUGc--AUAGUCCGUG-GCCGac -5' |
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| 17514 | 3' | -58.3 | NC_004456.1 | + | 21169 | 0.69 | 0.332278 |
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Target: 5'- aGUGccGCCugGaacaAUCAGGuCACCGGCa- -3' miRNA: 3'- gCAC--CGGugCa---UAGUCC-GUGGCCGac -5' |
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| 17514 | 3' | -58.3 | NC_004456.1 | + | 322 | 0.69 | 0.293738 |
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Target: 5'- uGUGGacCCAgGUGUCAGGUgaGCUGGCc- -3' miRNA: 3'- gCACC--GGUgCAUAGUCCG--UGGCCGac -5' |
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| 17514 | 3' | -58.3 | NC_004456.1 | + | 35730 | 0.71 | 0.252204 |
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Target: 5'- aCGUGGCCACGguuuccugGUacaCAGGUgaaagcuguACCGGCUc -3' miRNA: 3'- -GCACCGGUGCa-------UA---GUCCG---------UGGCCGAc -5' |
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| 17514 | 3' | -58.3 | NC_004456.1 | + | 35677 | 1.08 | 0.00044 |
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Target: 5'- cCGUGGCCACGUAUCAGGCACCGGCUGu -3' miRNA: 3'- -GCACCGGUGCAUAGUCCGUGGCCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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