miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17514 5' -52.6 NC_004456.1 + 13647 0.66 0.763142
Target:  5'- -gCAACCaaGC-UCGAUACaAGCCUUGg -3'
miRNA:   3'- gaGUUGGc-CGuAGCUAUGgUCGGAAC- -5'
17514 5' -52.6 NC_004456.1 + 35169 0.67 0.730631
Target:  5'- aCUgGuACCGGCAcUGGUAgCGGCCUg- -3'
miRNA:   3'- -GAgU-UGGCCGUaGCUAUgGUCGGAac -5'
17514 5' -52.6 NC_004456.1 + 31766 0.67 0.719562
Target:  5'- gCUCAAgCggGGCGUCcAUGCCcccuGCCUUGa -3'
miRNA:   3'- -GAGUUgG--CCGUAGcUAUGGu---CGGAAC- -5'
17514 5' -52.6 NC_004456.1 + 15380 0.69 0.605766
Target:  5'- -gCAGCCcGCAUCGuUACUAGCCcagUGg -3'
miRNA:   3'- gaGUUGGcCGUAGCuAUGGUCGGa--AC- -5'
17514 5' -52.6 NC_004456.1 + 27956 0.72 0.441824
Target:  5'- -cUAACUGGCAUCGccacCCAGCCUa- -3'
miRNA:   3'- gaGUUGGCCGUAGCuau-GGUCGGAac -5'
17514 5' -52.6 NC_004456.1 + 35778 0.72 0.441824
Target:  5'- --aGGCUGGUAUCGAUGCCGGUUg-- -3'
miRNA:   3'- gagUUGGCCGUAGCUAUGGUCGGaac -5'
17514 5' -52.6 NC_004456.1 + 35712 1.1 0.00114
Target:  5'- cCUCAACCGGCAUCGAUACCAGCCUUGg -3'
miRNA:   3'- -GAGUUGGCCGUAGCUAUGGUCGGAAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.