Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17514 | 5' | -52.6 | NC_004456.1 | + | 13647 | 0.66 | 0.763142 |
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Target: 5'- -gCAACCaaGC-UCGAUACaAGCCUUGg -3' miRNA: 3'- gaGUUGGc-CGuAGCUAUGgUCGGAAC- -5' |
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| 17514 | 5' | -52.6 | NC_004456.1 | + | 35169 | 0.67 | 0.730631 |
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Target: 5'- aCUgGuACCGGCAcUGGUAgCGGCCUg- -3' miRNA: 3'- -GAgU-UGGCCGUaGCUAUgGUCGGAac -5' |
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| 17514 | 5' | -52.6 | NC_004456.1 | + | 31766 | 0.67 | 0.719562 |
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Target: 5'- gCUCAAgCggGGCGUCcAUGCCcccuGCCUUGa -3' miRNA: 3'- -GAGUUgG--CCGUAGcUAUGGu---CGGAAC- -5' |
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| 17514 | 5' | -52.6 | NC_004456.1 | + | 15380 | 0.69 | 0.605766 |
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Target: 5'- -gCAGCCcGCAUCGuUACUAGCCcagUGg -3' miRNA: 3'- gaGUUGGcCGUAGCuAUGGUCGGa--AC- -5' |
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| 17514 | 5' | -52.6 | NC_004456.1 | + | 27956 | 0.72 | 0.441824 |
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Target: 5'- -cUAACUGGCAUCGccacCCAGCCUa- -3' miRNA: 3'- gaGUUGGCCGUAGCuau-GGUCGGAac -5' |
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| 17514 | 5' | -52.6 | NC_004456.1 | + | 35778 | 0.72 | 0.441824 |
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Target: 5'- --aGGCUGGUAUCGAUGCCGGUUg-- -3' miRNA: 3'- gagUUGGCCGUAGCUAUGGUCGGaac -5' |
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| 17514 | 5' | -52.6 | NC_004456.1 | + | 35712 | 1.1 | 0.00114 |
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Target: 5'- cCUCAACCGGCAUCGAUACCAGCCUUGg -3' miRNA: 3'- -GAGUUGGCCGUAGCUAUGGUCGGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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