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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17516 | 5' | -49.2 | NC_004456.1 | + | 12337 | 0.66 | 0.919479 |
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Target: 5'- aGCAgAGUGUCuugacuucucuGCACCAggaaauuaCCCUGUa -3' miRNA: 3'- -CGUaUCACAG-----------UGUGGUaaa-----GGGACGg -5' |
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| 17516 | 5' | -49.2 | NC_004456.1 | + | 21886 | 0.67 | 0.910141 |
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Target: 5'- cGCcaAGUGUUGCGCCAUguagaagacaaCCUGCg -3' miRNA: 3'- -CGuaUCACAGUGUGGUAaag--------GGACGg -5' |
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| 17516 | 5' | -49.2 | NC_004456.1 | + | 17520 | 0.67 | 0.898729 |
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Target: 5'- aCAUGGUGUCGUAauaGUggCCCUGCa -3' miRNA: 3'- cGUAUCACAGUGUgg-UAaaGGGACGg -5' |
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| 17516 | 5' | -49.2 | NC_004456.1 | + | 41310 | 0.69 | 0.790569 |
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Target: 5'- aGC-UGGUGggcCGCACCGUUaUCaCCaUGCCa -3' miRNA: 3'- -CGuAUCACa--GUGUGGUAA-AG-GG-ACGG- -5' |
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| 17516 | 5' | -49.2 | NC_004456.1 | + | 4164 | 0.7 | 0.758545 |
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Target: 5'- -gAUAGUcaGUUACACCAcgagCCCUGgCCa -3' miRNA: 3'- cgUAUCA--CAGUGUGGUaaa-GGGAC-GG- -5' |
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| 17516 | 5' | -49.2 | NC_004456.1 | + | 33403 | 0.71 | 0.679353 |
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Target: 5'- aGCcggAGUGUC-CACCAaggaCCUUGCCg -3' miRNA: 3'- -CGua-UCACAGuGUGGUaaa-GGGACGG- -5' |
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| 17516 | 5' | -49.2 | NC_004456.1 | + | 1901 | 0.79 | 0.303663 |
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Target: 5'- cGCAgcUGGUGUCaaugcgGCACCAg--CCCUGCUa -3' miRNA: 3'- -CGU--AUCACAG------UGUGGUaaaGGGACGG- -5' |
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| 17516 | 5' | -49.2 | NC_004456.1 | + | 40282 | 1.16 | 0.001039 |
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Target: 5'- uGCAUAGUGUCACACCAUUUCCCUGCCa -3' miRNA: 3'- -CGUAUCACAGUGUGGUAAAGGGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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