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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17517 | 3' | -64.4 | NC_004456.1 | + | 38482 | 0.66 | 0.255206 |
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Target: 5'- cGGCGAGUCUuCCuuUGGCCcaGCCUuguGCUGg -3' miRNA: 3'- -CCGCUCAGGcGG--ACCGG--UGGG---CGGCa -5' |
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| 17517 | 3' | -64.4 | NC_004456.1 | + | 8480 | 0.66 | 0.246549 |
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Target: 5'- gGGCGGGUCacuacccgcaccgGCCUgucuucccaGGCUGCCUGCUGa -3' miRNA: 3'- -CCGCUCAGg------------CGGA---------CCGGUGGGCGGCa -5' |
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| 17517 | 3' | -64.4 | NC_004456.1 | + | 36176 | 0.67 | 0.225405 |
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Target: 5'- aGGCGGGUUaaggcaaguggaUGCCaUGGCCGCUCagGCUGg -3' miRNA: 3'- -CCGCUCAG------------GCGG-ACCGGUGGG--CGGCa -5' |
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| 17517 | 3' | -64.4 | NC_004456.1 | + | 2790 | 0.67 | 0.225405 |
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Target: 5'- uGGCuuGUCCGCCuguucUGGCCugCUuuGCCu- -3' miRNA: 3'- -CCGcuCAGGCGG-----ACCGGugGG--CGGca -5' |
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| 17517 | 3' | -64.4 | NC_004456.1 | + | 41209 | 1.08 | 0.000117 |
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Target: 5'- uGGCGAGUCCGCCUGGCCACCCGCCGUa -3' miRNA: 3'- -CCGCUCAGGCGGACCGGUGGGCGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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