Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17517 | 5' | -56.7 | NC_004456.1 | + | 22339 | 0.66 | 0.557945 |
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Target: 5'- uUGAguugGAUGcUGCGGCCCAgCCuGCUg- -3' miRNA: 3'- -ACUa---UUGCcACGCCGGGU-GGuCGAgg -5' |
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| 17517 | 5' | -56.7 | NC_004456.1 | + | 39074 | 0.68 | 0.414193 |
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Target: 5'- uUGAUGGCG--GCGGCCUgcuuGCCAGUgaagCCc -3' miRNA: 3'- -ACUAUUGCcaCGCCGGG----UGGUCGa---GG- -5' |
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| 17517 | 5' | -56.7 | NC_004456.1 | + | 18627 | 0.68 | 0.414193 |
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Target: 5'- uUGAUAACGGUGCGaaCgACCAGggCUu -3' miRNA: 3'- -ACUAUUGCCACGCcgGgUGGUCgaGG- -5' |
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| 17517 | 5' | -56.7 | NC_004456.1 | + | 6554 | 0.71 | 0.287423 |
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Target: 5'- aGAUAuUGGUGCGGUCCgucaaugAUCAGCUgCu -3' miRNA: 3'- aCUAUuGCCACGCCGGG-------UGGUCGAgG- -5' |
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| 17517 | 5' | -56.7 | NC_004456.1 | + | 25306 | 0.72 | 0.266876 |
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Target: 5'- aUGAU-GCGGggaaucgGUGGCCgUGCCAGCUCa -3' miRNA: 3'- -ACUAuUGCCa------CGCCGG-GUGGUCGAGg -5' |
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| 17517 | 5' | -56.7 | NC_004456.1 | + | 7857 | 0.76 | 0.131964 |
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Target: 5'- cGAUucuACGGcgGCGGCCaugaugGCCAGUUCCa -3' miRNA: 3'- aCUAu--UGCCa-CGCCGGg-----UGGUCGAGG- -5' |
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| 17517 | 5' | -56.7 | NC_004456.1 | + | 41244 | 1.12 | 0.000289 |
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Target: 5'- gUGAUAACGGUGCGGCCCACCAGCUCCg -3' miRNA: 3'- -ACUAUUGCCACGCCGGGUGGUCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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