Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1758 | 5' | -56 | NC_001347.2 | + | 229051 | 0.66 | 0.960904 |
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Target: 5'- cGG-CGUGGUGu--GGGUGcuguuUGUGUCUGu -3' miRNA: 3'- -CCgGCACCGCcauCCCAU-----ACACAGAC- -5' |
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| 1758 | 5' | -56 | NC_001347.2 | + | 229008 | 0.66 | 0.957379 |
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Target: 5'- uGCUGUGGUGuaAGGGUaacGUGUGUUg- -3' miRNA: 3'- cCGGCACCGCcaUCCCA---UACACAGac -5' |
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| 1758 | 5' | -56 | NC_001347.2 | + | 218964 | 0.66 | 0.957379 |
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Target: 5'- cGGUCGUGGUGGUGuuGGUAcaUGUGg--- -3' miRNA: 3'- -CCGGCACCGCCAUc-CCAU--ACACagac -5' |
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| 1758 | 5' | -56 | NC_001347.2 | + | 229532 | 0.66 | 0.953643 |
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Target: 5'- gGGgCGcGGCGGguGGGUGUGUGcCg- -3' miRNA: 3'- -CCgGCaCCGCCauCCCAUACACaGac -5' |
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| 1758 | 5' | -56 | NC_001347.2 | + | 204257 | 0.67 | 0.936521 |
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Target: 5'- -aCCGUGGCGuugAGGGUAUacUGUCUa -3' miRNA: 3'- ccGGCACCGCca-UCCCAUAc-ACAGAc -5' |
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| 1758 | 5' | -56 | NC_001347.2 | + | 181 | 0.68 | 0.897958 |
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Target: 5'- gGGCgUGUGGCGGgugugccggcGGGGUgugguggcgggGUGUGUCg- -3' miRNA: 3'- -CCG-GCACCGCCa---------UCCCA-----------UACACAGac -5' |
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| 1758 | 5' | -56 | NC_001347.2 | + | 229660 | 0.68 | 0.897958 |
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Target: 5'- gGGCgUGUGGCGGgugugccggcGGGGUgugguggcgggGUGUGUCg- -3' miRNA: 3'- -CCG-GCACCGCCa---------UCCCA-----------UACACAGac -5' |
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| 1758 | 5' | -56 | NC_001347.2 | + | 37168 | 0.72 | 0.735594 |
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Target: 5'- gGGCgGUGGCGGccuUGGcGGUcgG-GUCUGu -3' miRNA: 3'- -CCGgCACCGCC---AUC-CCAuaCaCAGAC- -5' |
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| 1758 | 5' | -56 | NC_001347.2 | + | 140642 | 0.76 | 0.482846 |
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Target: 5'- cGGUCGUGGUGGUGGGGgugGUGg--- -3' miRNA: 3'- -CCGGCACCGCCAUCCCauaCACagac -5' |
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| 1758 | 5' | -56 | NC_001347.2 | + | 56248 | 1.11 | 0.003502 |
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Target: 5'- aGGCCGUGGCGGUAGGGUAUGUGUCUGa -3' miRNA: 3'- -CCGGCACCGCCAUCCCAUACACAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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