Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17589 | 3' | -58.8 | NC_004466.2 | + | 1087 | 0.66 | 0.497374 |
Target: 5'- uGGggUAUCCCCCgguuaUAGGcccuggaucaaAGgcaaCCCCCGa -3' miRNA: 3'- cCCuuAUAGGGGG-----GUCC-----------UCa---GGGGGU- -5' |
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17589 | 3' | -58.8 | NC_004466.2 | + | 33780 | 0.66 | 0.477144 |
Target: 5'- aGGGGAUAUCCgcgUUCCAauggucugcaacGGGGUCCCauCCAc -3' miRNA: 3'- -CCCUUAUAGG---GGGGU------------CCUCAGGG--GGU- -5' |
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17589 | 3' | -58.8 | NC_004466.2 | + | 25415 | 0.67 | 0.428458 |
Target: 5'- cGGGggUAUgCCCUuuGGGcugCCCCUAc -3' miRNA: 3'- -CCCuuAUA-GGGGggUCCucaGGGGGU- -5' |
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17589 | 3' | -58.8 | NC_004466.2 | + | 40843 | 0.67 | 0.419076 |
Target: 5'- uGGGGcUGaaCCCCUcGGAGUCCCUg- -3' miRNA: 3'- -CCCUuAUagGGGGGuCCUCAGGGGgu -5' |
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17589 | 3' | -58.8 | NC_004466.2 | + | 38878 | 0.68 | 0.365505 |
Target: 5'- aGGAGUAa-CCCCCAGGAugaaGUCCCaggugCCAu -3' miRNA: 3'- cCCUUAUagGGGGGUCCU----CAGGG-----GGU- -5' |
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17589 | 3' | -58.8 | NC_004466.2 | + | 35597 | 0.69 | 0.332509 |
Target: 5'- cGGGAcGUGUUCCaCCUGGGAcUCCCuCCGg -3' miRNA: 3'- -CCCU-UAUAGGG-GGGUCCUcAGGG-GGU- -5' |
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17589 | 3' | -58.8 | NC_004466.2 | + | 21636 | 0.7 | 0.292242 |
Target: 5'- cGGAAUAcuUCCUCCCAucuagccauuGGAGUagucgauggguuuaCCCCCAa -3' miRNA: 3'- cCCUUAU--AGGGGGGU----------CCUCA--------------GGGGGU- -5' |
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17589 | 3' | -58.8 | NC_004466.2 | + | 32131 | 0.7 | 0.266456 |
Target: 5'- uGGGGA-AUCCCCCgCAGGAGUacauacggCCUCGc -3' miRNA: 3'- -CCCUUaUAGGGGG-GUCCUCAg-------GGGGU- -5' |
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17589 | 3' | -58.8 | NC_004466.2 | + | 41627 | 0.7 | 0.266456 |
Target: 5'- aGGGAGgccaugAUCCaauCCgGGGAGgcuugCCCCCAu -3' miRNA: 3'- -CCCUUa-----UAGGg--GGgUCCUCa----GGGGGU- -5' |
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17589 | 3' | -58.8 | NC_004466.2 | + | 666 | 0.71 | 0.253301 |
Target: 5'- gGGGuuUGUCaagCCCCuGGGGuuUCCCCCGu -3' miRNA: 3'- -CCCuuAUAGg--GGGGuCCUC--AGGGGGU- -5' |
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17589 | 3' | -58.8 | NC_004466.2 | + | 41719 | 0.71 | 0.253301 |
Target: 5'- aGGAAgg--CCCCCAGGAG-CCCCg- -3' miRNA: 3'- cCCUUauagGGGGGUCCUCaGGGGgu -5' |
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17589 | 3' | -58.8 | NC_004466.2 | + | 1069 | 0.74 | 0.153499 |
Target: 5'- uGGGggUGgccuaUCCCCAGGGGUauagCCCCUAg -3' miRNA: 3'- -CCCuuAUag---GGGGGUCCUCA----GGGGGU- -5' |
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17589 | 3' | -58.8 | NC_004466.2 | + | 1103 | 1.1 | 0.000309 |
Target: 5'- gGGGAAUAUCCCCCCAGGAGUCCCCCAg -3' miRNA: 3'- -CCCUUAUAGGGGGGUCCUCAGGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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