Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17589 | 5' | -56.6 | NC_004466.2 | + | 30588 | 0.66 | 0.613981 |
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Target: 5'- --aGGCCU--UCCCAGGG--AUAGCUc -3' miRNA: 3'- ccaCCGGAuaGGGGUCCCcaUAUCGG- -5' |
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| 17589 | 5' | -56.6 | NC_004466.2 | + | 15194 | 0.66 | 0.581007 |
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Target: 5'- --cGGC---UCCCUGGGGGccuccUGUGGCCu -3' miRNA: 3'- ccaCCGgauAGGGGUCCCC-----AUAUCGG- -5' |
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| 17589 | 5' | -56.6 | NC_004466.2 | + | 14177 | 0.66 | 0.555974 |
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Target: 5'- uGGUGGCCUcgguucugcccuugGUgCCCUGGGuGGU---GCCu -3' miRNA: 3'- -CCACCGGA--------------UA-GGGGUCC-CCAuauCGG- -5' |
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| 17589 | 5' | -56.6 | NC_004466.2 | + | 31149 | 0.72 | 0.269125 |
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Target: 5'- cGG-GGUag--CCCCAGGGGUGgGGUCg -3' miRNA: 3'- -CCaCCGgauaGGGGUCCCCAUaUCGG- -5' |
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| 17589 | 5' | -56.6 | NC_004466.2 | + | 13820 | 0.76 | 0.149544 |
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Target: 5'- -aUGGCaguUCCCgAGcGGGUGUAGCCg -3' miRNA: 3'- ccACCGgauAGGGgUC-CCCAUAUCGG- -5' |
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| 17589 | 5' | -56.6 | NC_004466.2 | + | 1066 | 1.13 | 0.000324 |
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Target: 5'- gGGUGGCCUAUCCCCAGGGGUAUAGCCc -3' miRNA: 3'- -CCACCGGAUAGGGGUCCCCAUAUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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