Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17591 | 3' | -53.7 | NC_004466.2 | + | 31063 | 0.66 | 0.755572 |
Target: 5'- -uGAGGACGAGCUUUCGGcUAUGUcgauuGCu -3' miRNA: 3'- uuCUCCUGUUUGGGGGCCuAUGCA-----CG- -5' |
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17591 | 3' | -53.7 | NC_004466.2 | + | 945 | 0.66 | 0.722116 |
Target: 5'- cAAGGGGuu-GACCCCCGGAcuagccuUAUG-GUa -3' miRNA: 3'- -UUCUCCuguUUGGGGGCCU-------AUGCaCG- -5' |
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17591 | 3' | -53.7 | NC_004466.2 | + | 16961 | 0.67 | 0.701138 |
Target: 5'- -cGAGGGaguAACCCCUGGAcUGCuguccuaggugGUGCu -3' miRNA: 3'- uuCUCCUgu-UUGGGGGCCU-AUG-----------CACG- -5' |
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17591 | 3' | -53.7 | NC_004466.2 | + | 34531 | 0.67 | 0.689988 |
Target: 5'- -cGAGGAUGGAUgcucaaauccuUCCUGGA-ACGUGCa -3' miRNA: 3'- uuCUCCUGUUUG-----------GGGGCCUaUGCACG- -5' |
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17591 | 3' | -53.7 | NC_004466.2 | + | 38873 | 0.67 | 0.656233 |
Target: 5'- uAAGuAGGAguAACCCCCaGGAUGaagucccagGUGCc -3' miRNA: 3'- -UUC-UCCUguUUGGGGG-CCUAUg--------CACG- -5' |
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17591 | 3' | -53.7 | NC_004466.2 | + | 38672 | 0.68 | 0.595133 |
Target: 5'- uGAGGGGguaucaacucgauACGcuCCCCCgcguucuucacggaGGGUACGUGCu -3' miRNA: 3'- -UUCUCC-------------UGUuuGGGGG--------------CCUAUGCACG- -5' |
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17591 | 3' | -53.7 | NC_004466.2 | + | 776 | 0.69 | 0.570427 |
Target: 5'- --aAGGacuaucagucccccaGCAAACCCCCGGAUAUGa-- -3' miRNA: 3'- uucUCC---------------UGUUUGGGGGCCUAUGCacg -5' |
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17591 | 3' | -53.7 | NC_004466.2 | + | 25498 | 0.72 | 0.380875 |
Target: 5'- gAAGAGGACAAGCCUgaGGAcguauUugGUGa -3' miRNA: 3'- -UUCUCCUGUUUGGGggCCU-----AugCACg -5' |
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17591 | 3' | -53.7 | NC_004466.2 | + | 41546 | 0.74 | 0.296977 |
Target: 5'- -uGGGGGCAAGCcuCCCCGGAUugGa-- -3' miRNA: 3'- uuCUCCUGUUUG--GGGGCCUAugCacg -5' |
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17591 | 3' | -53.7 | NC_004466.2 | + | 3363 | 1.09 | 0.001146 |
Target: 5'- aAAGAGGACAAACCCCCGGAUACGUGCc -3' miRNA: 3'- -UUCUCCUGUUUGGGGGCCUAUGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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