Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17592 | 3' | -51.4 | NC_004466.2 | + | 23427 | 0.66 | 0.867459 |
Target: 5'- --cGGGAGguguCCCUCAGgaaCCUCAcaagacUCCc -3' miRNA: 3'- guaUCCUUau--GGGAGUCg--GGAGU------AGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 31336 | 0.66 | 0.858951 |
Target: 5'- ----aGAAUACCCcuucaaGGCCCUCGacUCCc -3' miRNA: 3'- guaucCUUAUGGGag----UCGGGAGU--AGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 1905 | 0.66 | 0.850184 |
Target: 5'- --aAGGAAUugaaCCUCuAGCCUaaggCGUCCg -3' miRNA: 3'- guaUCCUUAug--GGAG-UCGGGa---GUAGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 42942 | 0.66 | 0.83191 |
Target: 5'- gAUAGGGAggaUACCUUUgauGCCUaCGUCCu -3' miRNA: 3'- gUAUCCUU---AUGGGAGu--CGGGaGUAGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 8953 | 0.66 | 0.828142 |
Target: 5'- gAUAGGAccACCUUCAGCCaugggacgauUCCg -3' miRNA: 3'- gUAUCCUuaUGGGAGUCGGgagu------AGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 21728 | 0.67 | 0.821463 |
Target: 5'- --cGGGcuuUACCCUCAGCagcacccCCUC-UCCc -3' miRNA: 3'- guaUCCuu-AUGGGAGUCG-------GGAGuAGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 13904 | 0.67 | 0.812719 |
Target: 5'- gGUAGcAAUACCCUCGGCUa-CAcCCg -3' miRNA: 3'- gUAUCcUUAUGGGAGUCGGgaGUaGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 37999 | 0.67 | 0.792699 |
Target: 5'- gGUAGGAGUcaucuaCCUCuauGCCCUCcUUCu -3' miRNA: 3'- gUAUCCUUAug----GGAGu--CGGGAGuAGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 41190 | 0.67 | 0.792699 |
Target: 5'- aGUAGGuguGUGCaugaCCUUGGCCCUCuUCg -3' miRNA: 3'- gUAUCCu--UAUG----GGAGUCGGGAGuAGg -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 25035 | 0.67 | 0.792699 |
Target: 5'- ---uGGAAcGCCCUagCAGCCCUagagcugguaAUCCa -3' miRNA: 3'- guauCCUUaUGGGA--GUCGGGAg---------UAGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 10910 | 0.68 | 0.771949 |
Target: 5'- --gGGGAAUgGCCCagAGCCUUCugagCCu -3' miRNA: 3'- guaUCCUUA-UGGGagUCGGGAGua--GG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 19198 | 0.68 | 0.76133 |
Target: 5'- --aAGGcuacACCCgCAGCCCcCAUCCc -3' miRNA: 3'- guaUCCuua-UGGGaGUCGGGaGUAGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 17022 | 0.68 | 0.76133 |
Target: 5'- --cAGGggUuacuACCCUCGGggucaagcuCCUUCAUCUg -3' miRNA: 3'- guaUCCuuA----UGGGAGUC---------GGGAGUAGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 38867 | 0.7 | 0.619441 |
Target: 5'- --aAGGAGUuccagaucaccaucuACCCUCAGCCCUgcuaCCu -3' miRNA: 3'- guaUCCUUA---------------UGGGAGUCGGGAgua-GG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 14861 | 0.73 | 0.470274 |
Target: 5'- --cAGGAAUACCUg-AGCCCUCuacagCCa -3' miRNA: 3'- guaUCCUUAUGGGagUCGGGAGua---GG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 26422 | 0.75 | 0.384651 |
Target: 5'- --aAGGAAUugcucccucagccuaGCCCcaUCGGCCCUCGUgCCa -3' miRNA: 3'- guaUCCUUA---------------UGGG--AGUCGGGAGUA-GG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 24084 | 0.75 | 0.380926 |
Target: 5'- --gAGGAAUGCCgUaccgUAGCCCUCAagcuUCCa -3' miRNA: 3'- guaUCCUUAUGGgA----GUCGGGAGU----AGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 37026 | 0.75 | 0.362664 |
Target: 5'- aGUGGGcAUACgaCUCGGCCCUCAaggcuuacUCCg -3' miRNA: 3'- gUAUCCuUAUGg-GAGUCGGGAGU--------AGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 24899 | 0.8 | 0.181089 |
Target: 5'- -cUGGGAAUGgUCUUAGCCCUCGgaUCCa -3' miRNA: 3'- guAUCCUUAUgGGAGUCGGGAGU--AGG- -5' |
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17592 | 3' | -51.4 | NC_004466.2 | + | 3691 | 1.12 | 0.001147 |
Target: 5'- gCAUAGGAAUACCCUCAGCCCUCAUCCu -3' miRNA: 3'- -GUAUCCUUAUGGGAGUCGGGAGUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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