Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17592 | 5' | -50.7 | NC_004466.2 | + | 14532 | 0.66 | 0.899321 |
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Target: 5'- -aGGAGA----GCCGcGAGU-GCUGCu -3' miRNA: 3'- gaCCUCUcauaUGGCuCUCAuCGACG- -5' |
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| 17592 | 5' | -50.7 | NC_004466.2 | + | 38712 | 0.66 | 0.876234 |
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Target: 5'- -cGGAGGGUacGUGCUGAG-GaAGCaGCu -3' miRNA: 3'- gaCCUCUCA--UAUGGCUCuCaUCGaCG- -5' |
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| 17592 | 5' | -50.7 | NC_004466.2 | + | 3212 | 0.67 | 0.832321 |
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Target: 5'- aCUGGAGGaUGUcCCGAGuAGUAuCUGUg -3' miRNA: 3'- -GACCUCUcAUAuGGCUC-UCAUcGACG- -5' |
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| 17592 | 5' | -50.7 | NC_004466.2 | + | 13835 | 0.68 | 0.812076 |
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Target: 5'- ----cGGGUGUaGCCGAGGGUauugcuaccgcuaGGCUGCu -3' miRNA: 3'- gaccuCUCAUA-UGGCUCUCA-------------UCGACG- -5' |
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| 17592 | 5' | -50.7 | NC_004466.2 | + | 13099 | 0.68 | 0.782634 |
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Target: 5'- -aGGAGAGUggguAUGCUGGG-GU-GUUGCa -3' miRNA: 3'- gaCCUCUCA----UAUGGCUCuCAuCGACG- -5' |
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| 17592 | 5' | -50.7 | NC_004466.2 | + | 36887 | 0.69 | 0.761468 |
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Target: 5'- cCUGGAGAa---GCUGGGGGUGGCUc- -3' miRNA: 3'- -GACCUCUcauaUGGCUCUCAUCGAcg -5' |
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| 17592 | 5' | -50.7 | NC_004466.2 | + | 36204 | 0.72 | 0.534537 |
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Target: 5'- gCUGGAGGGUu--CCGAuGAGUGGCc-- -3' miRNA: 3'- -GACCUCUCAuauGGCU-CUCAUCGacg -5' |
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| 17592 | 5' | -50.7 | NC_004466.2 | + | 27773 | 0.75 | 0.388989 |
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Target: 5'- cCUGGAGGGUAUcuCCGAGGau-GCUGUa -3' miRNA: 3'- -GACCUCUCAUAu-GGCUCUcauCGACG- -5' |
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| 17592 | 5' | -50.7 | NC_004466.2 | + | 3656 | 1.13 | 0.0013 |
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Target: 5'- gCUGGAGAGUAUACCGAGAGUAGCUGCu -3' miRNA: 3'- -GACCUCUCAUAUGGCUCUCAUCGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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