Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17593 | 5' | -44.5 | NC_004466.2 | + | 36189 | 0.66 | 0.998087 |
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Target: 5'- uGGGAGUUCcUCGugggcuuGCAg-CUCGGGa -3' miRNA: 3'- -CCUUCAAGaAGCuau----UGUagGAGUCC- -5' |
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| 17593 | 5' | -44.5 | NC_004466.2 | + | 15342 | 0.66 | 0.998087 |
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Target: 5'- cGGAGacCUUCGAUAGCAcCCUguuGGa -3' miRNA: 3'- cCUUCaaGAAGCUAUUGUaGGAgu-CC- -5' |
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| 17593 | 5' | -44.5 | NC_004466.2 | + | 37783 | 0.67 | 0.997169 |
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Target: 5'- aGGccGAGUUCcUCGAUAGCccggcaGUCCUUGGu -3' miRNA: 3'- -CC--UUCAAGaAGCUAUUG------UAGGAGUCc -5' |
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| 17593 | 5' | -44.5 | NC_004466.2 | + | 25397 | 0.67 | 0.997005 |
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Target: 5'- aGGAGcaUCUUCGucaucgAaguccucaccaaauACGUCCUCAGGc -3' miRNA: 3'- -CCUUcaAGAAGCua----U--------------UGUAGGAGUCC- -5' |
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| 17593 | 5' | -44.5 | NC_004466.2 | + | 18223 | 0.71 | 0.962304 |
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Target: 5'- cGAAGUaaucccggaucUCUUgGAUGAgGUCUUUAGGg -3' miRNA: 3'- cCUUCA-----------AGAAgCUAUUgUAGGAGUCC- -5' |
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| 17593 | 5' | -44.5 | NC_004466.2 | + | 9143 | 0.74 | 0.875636 |
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Target: 5'- aGAAGUUCUUgGGUAAgGUCCUgAcGGu -3' miRNA: 3'- cCUUCAAGAAgCUAUUgUAGGAgU-CC- -5' |
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| 17593 | 5' | -44.5 | NC_004466.2 | + | 42939 | 0.75 | 0.839844 |
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Target: 5'- aGGGAGgauacCUUUGAUGccuACGUCCUCAaGGg -3' miRNA: 3'- -CCUUCaa---GAAGCUAU---UGUAGGAGU-CC- -5' |
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| 17593 | 5' | -44.5 | NC_004466.2 | + | 5087 | 1.16 | 0.004916 |
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Target: 5'- uGGAAGUUCUUCGAUAACAUCCUCAGGa -3' miRNA: 3'- -CCUUCAAGAAGCUAUUGUAGGAGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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