Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17595 | 3' | -59.1 | NC_004466.2 | + | 18971 | 0.66 | 0.462506 |
Target: 5'- uGACC-CCACCUUCG-CUGacaAGCCUa -3' miRNA: 3'- uCUGGuGGUGGGGGCuGAUg--UCGGAc -5' |
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17595 | 3' | -59.1 | NC_004466.2 | + | 26294 | 0.66 | 0.452651 |
Target: 5'- uGGACCAUCACCUCUccaGACUcacuACGGCg-- -3' miRNA: 3'- -UCUGGUGGUGGGGG---CUGA----UGUCGgac -5' |
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17595 | 3' | -59.1 | NC_004466.2 | + | 43208 | 0.66 | 0.433291 |
Target: 5'- cGAUCACUucGCCCuuGcUUACAGCCa- -3' miRNA: 3'- uCUGGUGG--UGGGggCuGAUGUCGGac -5' |
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17595 | 3' | -59.1 | NC_004466.2 | + | 37287 | 0.67 | 0.399695 |
Target: 5'- cGACCGaugCACCCCCGACccuucguaccgcugGCGGUCa- -3' miRNA: 3'- uCUGGUg--GUGGGGGCUGa-------------UGUCGGac -5' |
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17595 | 3' | -59.1 | NC_004466.2 | + | 1866 | 0.67 | 0.378243 |
Target: 5'- cGACCACCAuuaacUCCUCGuaGCU-CAGUCUGg -3' miRNA: 3'- uCUGGUGGU-----GGGGGC--UGAuGUCGGAC- -5' |
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17595 | 3' | -59.1 | NC_004466.2 | + | 36285 | 0.67 | 0.378243 |
Target: 5'- uGGCCACCGCaagcgugaucuUCCCGAgCUGCAaGCCc- -3' miRNA: 3'- uCUGGUGGUG-----------GGGGCU-GAUGU-CGGac -5' |
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17595 | 3' | -59.1 | NC_004466.2 | + | 39260 | 0.7 | 0.236824 |
Target: 5'- aGGACCACUACCCCgGA----GGUCUGg -3' miRNA: 3'- -UCUGGUGGUGGGGgCUgaugUCGGAC- -5' |
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17595 | 3' | -59.1 | NC_004466.2 | + | 675 | 0.74 | 0.134844 |
Target: 5'- uAGGCCACCAuCCCCCGAguCUACGGaCa- -3' miRNA: 3'- -UCUGGUGGU-GGGGGCU--GAUGUCgGac -5' |
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17595 | 3' | -59.1 | NC_004466.2 | + | 24916 | 0.75 | 0.110997 |
Target: 5'- uAGACCugggggaaACCCCaGGCUACAGCCUGa -3' miRNA: 3'- -UCUGGugg-----UGGGGgCUGAUGUCGGAC- -5' |
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17595 | 3' | -59.1 | NC_004466.2 | + | 15399 | 0.8 | 0.047118 |
Target: 5'- uGGgCAUCAgCCUCGACUACAGCCUGg -3' miRNA: 3'- uCUgGUGGUgGGGGCUGAUGUCGGAC- -5' |
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17595 | 3' | -59.1 | NC_004466.2 | + | 5995 | 1.09 | 0.000328 |
Target: 5'- gAGACCACCACCCCCGACUACAGCCUGg -3' miRNA: 3'- -UCUGGUGGUGGGGGCUGAUGUCGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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