Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17595 | 5' | -52.8 | NC_004466.2 | + | 9172 | 0.66 | 0.793812 |
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Target: 5'- aCCCuGAUG-GCcUUuGGUGGAgGCAUCg -3' miRNA: 3'- -GGGuCUAUgCGuAGuCCACCU-CGUAG- -5' |
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| 17595 | 5' | -52.8 | NC_004466.2 | + | 37208 | 0.68 | 0.686003 |
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Target: 5'- gCCAGcgGUACGaaggGUCGGG-GGuGCAUCg -3' miRNA: 3'- gGGUC--UAUGCg---UAGUCCaCCuCGUAG- -5' |
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| 17595 | 5' | -52.8 | NC_004466.2 | + | 37964 | 0.68 | 0.663375 |
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Target: 5'- cCCCAGGcccaaagccUugGCcaucgucuccGUCAGGUaGGAGuCAUCu -3' miRNA: 3'- -GGGUCU---------AugCG----------UAGUCCA-CCUC-GUAG- -5' |
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| 17595 | 5' | -52.8 | NC_004466.2 | + | 11218 | 0.69 | 0.606457 |
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Target: 5'- cCCCAGAUG---GUCuGGUGGAGUAccUCa -3' miRNA: 3'- -GGGUCUAUgcgUAGuCCACCUCGU--AG- -5' |
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| 17595 | 5' | -52.8 | NC_004466.2 | + | 44071 | 0.69 | 0.595103 |
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Target: 5'- aCCGGAccUAUgGUcgCAGGcUGGGGCGUCu -3' miRNA: 3'- gGGUCU--AUG-CGuaGUCC-ACCUCGUAG- -5' |
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| 17595 | 5' | -52.8 | NC_004466.2 | + | 32693 | 0.7 | 0.539018 |
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Target: 5'- aCCCAGG---GCAUC--GUGGGGCGUCg -3' miRNA: 3'- -GGGUCUaugCGUAGucCACCUCGUAG- -5' |
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| 17595 | 5' | -52.8 | NC_004466.2 | + | 11896 | 0.71 | 0.484844 |
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Target: 5'- cCCCAGAccgACGUAUCgguaucccagGGGuUGGAGuCGUCa -3' miRNA: 3'- -GGGUCUa--UGCGUAG----------UCC-ACCUC-GUAG- -5' |
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| 17595 | 5' | -52.8 | NC_004466.2 | + | 5961 | 1.11 | 0.000904 |
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Target: 5'- uCCCAGAUACGCAUCAGGUGGAGCAUCu -3' miRNA: 3'- -GGGUCUAUGCGUAGUCCACCUCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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