Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17596 | 5' | -46.4 | NC_004466.2 | + | 35455 | 0.66 | 0.982806 |
Target: 5'- gAGACAGCGCc-GACUccgugAGCaAGAACg -3' miRNA: 3'- -UCUGUCGUGauCUGAua---UCGgUUUUG- -5' |
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17596 | 5' | -46.4 | NC_004466.2 | + | 27825 | 0.66 | 0.982806 |
Target: 5'- uGGAgCAGCAauggAGACUGga-CCGAAGCg -3' miRNA: 3'- -UCU-GUCGUga--UCUGAUaucGGUUUUG- -5' |
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17596 | 5' | -46.4 | NC_004466.2 | + | 15322 | 0.66 | 0.980387 |
Target: 5'- -aAUGGC-CcAGGCUGUAGUCGAGGCu -3' miRNA: 3'- ucUGUCGuGaUCUGAUAUCGGUUUUG- -5' |
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17596 | 5' | -46.4 | NC_004466.2 | + | 15758 | 0.67 | 0.974799 |
Target: 5'- cAGGCuGCugUAGAacg-GGCCAAAc- -3' miRNA: 3'- -UCUGuCGugAUCUgauaUCGGUUUug -5' |
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17596 | 5' | -46.4 | NC_004466.2 | + | 24829 | 0.67 | 0.968134 |
Target: 5'- -aGCAGCAUccgucAGGCUGUAGCCu---- -3' miRNA: 3'- ucUGUCGUGa----UCUGAUAUCGGuuuug -5' |
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17596 | 5' | -46.4 | NC_004466.2 | + | 36981 | 0.67 | 0.968134 |
Target: 5'- aAGGCGGC-CUGGAUgac-GCCAucGCa -3' miRNA: 3'- -UCUGUCGuGAUCUGauauCGGUuuUG- -5' |
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17596 | 5' | -46.4 | NC_004466.2 | + | 40292 | 0.67 | 0.964369 |
Target: 5'- cAGAcCGGCACccaGGGCgcUAGCCGAGGu -3' miRNA: 3'- -UCU-GUCGUGa--UCUGauAUCGGUUUUg -5' |
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17596 | 5' | -46.4 | NC_004466.2 | + | 29091 | 0.67 | 0.960304 |
Target: 5'- -aGCAGCAUUAGAagaGUGGCUAcGGACa -3' miRNA: 3'- ucUGUCGUGAUCUga-UAUCGGU-UUUG- -5' |
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17596 | 5' | -46.4 | NC_004466.2 | + | 12876 | 0.69 | 0.909169 |
Target: 5'- -cACAGCAguUUGGAUUGUAGCCcgauuaAGAACa -3' miRNA: 3'- ucUGUCGU--GAUCUGAUAUCGG------UUUUG- -5' |
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17596 | 5' | -46.4 | NC_004466.2 | + | 24849 | 0.7 | 0.89416 |
Target: 5'- gAGugAGUACUAuGACUGgaagGGCUacGAAGCu -3' miRNA: 3'- -UCugUCGUGAU-CUGAUa---UCGG--UUUUG- -5' |
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17596 | 5' | -46.4 | NC_004466.2 | + | 15833 | 0.71 | 0.860346 |
Target: 5'- cAGGCugaaacCGCUAGAC-GUGGCCAGGAUa -3' miRNA: 3'- -UCUGuc----GUGAUCUGaUAUCGGUUUUG- -5' |
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17596 | 5' | -46.4 | NC_004466.2 | + | 6511 | 1.12 | 0.003408 |
Target: 5'- cAGACAGCACUAGACUAUAGCCAAAACg -3' miRNA: 3'- -UCUGUCGUGAUCUGAUAUCGGUUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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