Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17599 | 3' | -51.5 | NC_004466.2 | + | 13840 | 0.66 | 0.850189 |
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Target: 5'- gUAGCCGAGGGuauugCUACCGcuagGCUGCUc -3' miRNA: 3'- -GUUGGCUCCUug---GAUGGCag--UGAUGG- -5' |
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| 17599 | 3' | -51.5 | NC_004466.2 | + | 19892 | 0.67 | 0.841298 |
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Target: 5'- uGGuuGAGGccCCUugCGgUACUGCCu -3' miRNA: 3'- gUUggCUCCuuGGAugGCaGUGAUGG- -5' |
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| 17599 | 3' | -51.5 | NC_004466.2 | + | 36434 | 0.67 | 0.832174 |
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Target: 5'- -uACCGGGuacaGGCUU-CCGUUGCUGCCa -3' miRNA: 3'- guUGGCUCc---UUGGAuGGCAGUGAUGG- -5' |
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| 17599 | 3' | -51.5 | NC_004466.2 | + | 45164 | 0.67 | 0.832173 |
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Target: 5'- aAGCCGAgcaagugcGGuAACuCUGCCGUCAUcACUg -3' miRNA: 3'- gUUGGCU--------CC-UUG-GAUGGCAGUGaUGG- -5' |
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| 17599 | 3' | -51.5 | NC_004466.2 | + | 33113 | 0.69 | 0.741335 |
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Target: 5'- --uCCGAGGGuucgACCUACCGUg---GCCg -3' miRNA: 3'- guuGGCUCCU----UGGAUGGCAgugaUGG- -5' |
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| 17599 | 3' | -51.5 | NC_004466.2 | + | 34152 | 0.73 | 0.50716 |
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Target: 5'- gAGCCGcAGGAAgCUACCGcaagcaUCACaGCCu -3' miRNA: 3'- gUUGGC-UCCUUgGAUGGC------AGUGaUGG- -5' |
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| 17599 | 3' | -51.5 | NC_004466.2 | + | 19997 | 0.76 | 0.325514 |
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Target: 5'- gAACCGAcgGGggUCUACCcggaacUCACUACCg -3' miRNA: 3'- gUUGGCU--CCuuGGAUGGc-----AGUGAUGG- -5' |
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| 17599 | 3' | -51.5 | NC_004466.2 | + | 9954 | 1.14 | 0.001055 |
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Target: 5'- aCAACCGAGGAACCUACCGUCACUACCg -3' miRNA: 3'- -GUUGGCUCCUUGGAUGGCAGUGAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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