Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
176 | 5' | -65.9 | AC_000007.1 | + | 18601 | 0.71 | 0.068755 |
Target: 5'- aGCCGCGCgucccuGCGCCguGCCgccaGCGGuCCGc -3' miRNA: 3'- aCGGCGCG------UGUGGguCGGg---CGCC-GGU- -5' |
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176 | 5' | -65.9 | AC_000007.1 | + | 12246 | 0.71 | 0.065 |
Target: 5'- uUGCCaGCGCGCGCgggugccaccgCCAGCCCaggucCGGCCc -3' miRNA: 3'- -ACGG-CGCGUGUG-----------GGUCGGGc----GCCGGu -5' |
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176 | 5' | -65.9 | AC_000007.1 | + | 8268 | 0.71 | 0.060926 |
Target: 5'- cGCCGCGCGaGCCCAaaguccagauguccGCgCGCGGCg- -3' miRNA: 3'- aCGGCGCGUgUGGGU--------------CGgGCGCCGgu -5' |
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176 | 5' | -65.9 | AC_000007.1 | + | 12729 | 0.72 | 0.056458 |
Target: 5'- cUGCUGCGCGCGCUCAcgcuGCgCCaCGGCCu -3' miRNA: 3'- -ACGGCGCGUGUGGGU----CG-GGcGCCGGu -5' |
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176 | 5' | -65.9 | AC_000007.1 | + | 16233 | 0.72 | 0.054885 |
Target: 5'- -aCCGCGCACgucGCaCCGGCCgacggGCGGCCAu -3' miRNA: 3'- acGGCGCGUG---UG-GGUCGGg----CGCCGGU- -5' |
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176 | 5' | -65.9 | AC_000007.1 | + | 23899 | 1.07 | 0.000066 |
Target: 5'- gUGCCGCGCACACCCAGCCCGCGGCCAu -3' miRNA: 3'- -ACGGCGCGUGUGGGUCGGGCGCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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