Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17602 | 3' | -59.6 | NC_004466.2 | + | 33314 | 0.66 | 0.451178 |
Target: 5'- cGGcCAGGACgauCGGGGCCGgCACCAg- -3' miRNA: 3'- uCCcGUCUUG---GCUCCGGUgGUGGUgg -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 35470 | 0.66 | 0.431986 |
Target: 5'- cAGGGCAGcAGCCGccAGacCCAUCucacgGCCACCc -3' miRNA: 3'- -UCCCGUC-UUGGC--UCc-GGUGG-----UGGUGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 32773 | 0.66 | 0.431986 |
Target: 5'- cAGGG-AGAuCCccuGGCCACUAUgGCCa -3' miRNA: 3'- -UCCCgUCUuGGcu-CCGGUGGUGgUGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 25344 | 0.66 | 0.431986 |
Target: 5'- aGGGGCAGc-CCaaaGGGCaUACCcCCGCCu -3' miRNA: 3'- -UCCCGUCuuGGc--UCCG-GUGGuGGUGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 36947 | 0.66 | 0.41328 |
Target: 5'- aGGGGCA--GCCGAGGUacaACgUAUCAUCa -3' miRNA: 3'- -UCCCGUcuUGGCUCCGg--UG-GUGGUGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 17437 | 0.66 | 0.41328 |
Target: 5'- aGGGGCguuAGGAUCGguauGGGCUgaGCCugCugCa -3' miRNA: 3'- -UCCCG---UCUUGGC----UCCGG--UGGugGugG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 41046 | 0.66 | 0.41328 |
Target: 5'- cGGGaguGACaGGGGCCACaauCCACCa -3' miRNA: 3'- uCCCgucUUGgCUCCGGUGgu-GGUGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 14350 | 0.67 | 0.39868 |
Target: 5'- uGGGCAGGAgcggguacaguugagUCGucaAGGCUcCUACCGCCc -3' miRNA: 3'- uCCCGUCUU---------------GGC---UCCGGuGGUGGUGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 42374 | 0.67 | 0.386179 |
Target: 5'- cGGGGUAGAAcCCGcAGucaaCAUCGCCGCUg -3' miRNA: 3'- -UCCCGUCUU-GGC-UCcg--GUGGUGGUGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 16948 | 0.67 | 0.360277 |
Target: 5'- cAGGaaAGAACCuauGAGGgCACCACCcauacgACCa -3' miRNA: 3'- -UCCcgUCUUGG---CUCCgGUGGUGG------UGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 993 | 0.68 | 0.335612 |
Target: 5'- aGGGGCAu-ACCccuggggauAGGCCACCcCCACUu -3' miRNA: 3'- -UCCCGUcuUGGc--------UCCGGUGGuGGUGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 40256 | 0.68 | 0.319865 |
Target: 5'- cAGGaagaAGAGCCcguagaagaugGAGGCCACCAUCagACCg -3' miRNA: 3'- -UCCcg--UCUUGG-----------CUCCGGUGGUGG--UGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 6535 | 0.68 | 0.319865 |
Target: 5'- gAGGGC-GAAUgGaAGGCUACgGCCcCCa -3' miRNA: 3'- -UCCCGuCUUGgC-UCCGGUGgUGGuGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 32579 | 0.68 | 0.319865 |
Target: 5'- uGGGaCAGGauaGCCc-GGCCACgCACCACg -3' miRNA: 3'- uCCC-GUCU---UGGcuCCGGUG-GUGGUGg -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 17682 | 0.69 | 0.297297 |
Target: 5'- uGGGCGucaauacucCUGAGGCUACCGCCcuCCg -3' miRNA: 3'- uCCCGUcuu------GGCUCCGGUGGUGGu-GG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 13835 | 0.69 | 0.269155 |
Target: 5'- cGGGUguAGCCGAGGgUAUUGCUACCg -3' miRNA: 3'- uCCCGucUUGGCUCCgGUGGUGGUGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 11335 | 0.7 | 0.255909 |
Target: 5'- uAGGGUccguuGGAuCCcaaGAGGCCGggaCACCACCa -3' miRNA: 3'- -UCCCG-----UCUuGG---CUCCGGUg--GUGGUGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 727 | 0.7 | 0.247589 |
Target: 5'- aGGGGCuugacaaaccccgcGGAACCu-GGCCuaaugGCCACCACg -3' miRNA: 3'- -UCCCG--------------UCUUGGcuCCGG-----UGGUGGUGg -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 17092 | 0.72 | 0.188865 |
Target: 5'- uGGGGCAGcgauaacaccagcAcccgcaguaucuaguACCGAgaGGCCAgCACCACCu -3' miRNA: 3'- -UCCCGUC-------------U---------------UGGCU--CCGGUgGUGGUGG- -5' |
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17602 | 3' | -59.6 | NC_004466.2 | + | 32807 | 0.72 | 0.187368 |
Target: 5'- uGGGUAcGAACgaaGAuacGGCCACCACCAUUa -3' miRNA: 3'- uCCCGU-CUUGg--CU---CCGGUGGUGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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