miRNA: 3'- aACCAC--UGCCU-AUGCAAuCAuGGUGG- -5'
miRNA: 3'- aaCCaCUGCCUauGCA---AUCAUGGUGG- -5'
miRNA: 3'- aaCCACUGCCuauGCa----AUCAUGGUGG- -5'
miRNA: 3'- -AACCACUGCCUAUGCAAUCAUGGUGG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17602 | 5' | -49.4 | NC_004466.2 | + | 38514 | 0.67 | 0.909714 |
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Target: 5'- aUGGUGaaGCGGGcUGCGUU-GU-CCACUu -3' miRNA: 3'- aACCAC--UGCCU-AUGCAAuCAuGGUGG- -5' |
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| 17602 | 5' | -49.4 | NC_004466.2 | + | 37508 | 0.72 | 0.640619 |
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Target: 5'- -cGG-GAUGGGcuCGUcuuUAGUACCACCg -3' miRNA: 3'- aaCCaCUGCCUauGCA---AUCAUGGUGG- -5' |
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| 17602 | 5' | -49.4 | NC_004466.2 | + | 8193 | 0.73 | 0.582562 |
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Target: 5'- -aGGUGACGGuu-CGaacacUGGUACCGCUg -3' miRNA: 3'- aaCCACUGCCuauGCa----AUCAUGGUGG- -5' |
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| 17602 | 5' | -49.4 | NC_004466.2 | + | 14204 | 1.09 | 0.003026 |
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Target: 5'- cUUGGUGACGGAUACGUUAGUACCACCc -3' miRNA: 3'- -AACCACUGCCUAUGCAAUCAUGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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