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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17603 | 3' | -55.9 | NC_004466.2 | + | 19626 | 0.66 | 0.580413 |
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Target: 5'- cACCugGGCCGGcuGCUUCAGUuucaaCGUAa -3' miRNA: 3'- -UGGugCUGGUCauCGAAGUCG-----GCGUc -5' |
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| 17603 | 3' | -55.9 | NC_004466.2 | + | 17215 | 0.66 | 0.569319 |
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Target: 5'- aGCCAC-ACCAG-AGa--CAGCCGCAu -3' miRNA: 3'- -UGGUGcUGGUCaUCgaaGUCGGCGUc -5' |
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| 17603 | 3' | -55.9 | NC_004466.2 | + | 38132 | 0.67 | 0.514804 |
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Target: 5'- aACCACGGgguCCAGUuucuguagcAGgUUCGGCUGguGg -3' miRNA: 3'- -UGGUGCU---GGUCA---------UCgAAGUCGGCguC- -5' |
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| 17603 | 3' | -55.9 | NC_004466.2 | + | 15901 | 0.69 | 0.42279 |
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Target: 5'- gGCCACGuCUAGcGGUUUCAGCCuGgAGc -3' miRNA: 3'- -UGGUGCuGGUCaUCGAAGUCGG-CgUC- -5' |
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| 17603 | 3' | -55.9 | NC_004466.2 | + | 2281 | 0.74 | 0.208545 |
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Target: 5'- uCCugGACCgaGGUGGCUUCGGUgcuaaGCAGa -3' miRNA: 3'- uGGugCUGG--UCAUCGAAGUCGg----CGUC- -5' |
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| 17603 | 3' | -55.9 | NC_004466.2 | + | 14963 | 1.09 | 0.000591 |
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Target: 5'- cACCACGACCAGUAGCUUCAGCCGCAGg -3' miRNA: 3'- -UGGUGCUGGUCAUCGAAGUCGGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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