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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17604 | 5' | -58.1 | NC_004466.2 | + | 15202 | 0.66 | 0.480576 |
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Target: 5'- aGCC-CCUgagGUGGCC-UCUGAG--GCCg -3' miRNA: 3'- cCGGaGGA---CACCGGaAGACUCgaCGG- -5' |
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| 17604 | 5' | -58.1 | NC_004466.2 | + | 32477 | 0.66 | 0.470439 |
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Target: 5'- cGGUcaaCUUCUGUGGCCUggacuUCgGuGCUGUa -3' miRNA: 3'- -CCG---GAGGACACCGGA-----AGaCuCGACGg -5' |
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| 17604 | 5' | -58.1 | NC_004466.2 | + | 41655 | 0.66 | 0.460411 |
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Target: 5'- gGGgCUCCUGggGGCCUUcCUGAcggGUCa -3' miRNA: 3'- -CCgGAGGACa-CCGGAA-GACUcgaCGG- -5' |
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| 17604 | 5' | -58.1 | NC_004466.2 | + | 16747 | 0.69 | 0.312877 |
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Target: 5'- cGCCUCCUGggcauucuccucaGCCaUCUGAG-UGCCg -3' miRNA: 3'- cCGGAGGACac-----------CGGaAGACUCgACGG- -5' |
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| 17604 | 5' | -58.1 | NC_004466.2 | + | 35557 | 0.72 | 0.199747 |
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Target: 5'- uGUCUCCggGUGGCCgugagaugggUCUGGcggcGCUGCCc -3' miRNA: 3'- cCGGAGGa-CACCGGa---------AGACU----CGACGG- -5' |
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| 17604 | 5' | -58.1 | NC_004466.2 | + | 15183 | 1.12 | 0.000214 |
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Target: 5'- gGGCCUCCUGUGGCCUUCUGAGCUGCCc -3' miRNA: 3'- -CCGGAGGACACCGGAAGACUCGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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