miRNA: 3'- aCAaGUcUCGUGGGUGCaaCUAGG-GG- -5'
miRNA: 3'- -ACaAGUCuCGU-GGG-------------UGCAACUAGGGg -5'
miRNA: 3'- acaAG-UCUCGUGGG---UGCAACUaGGGG- -5'
miRNA: 3'- -ACAAGUCUCGUGGGUGCAACUAGGGG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17606 | 3' | -52.9 | NC_004466.2 | + | 42407 | 0.66 | 0.796792 |
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Target: 5'- aGUaUAcGGCACCCACG--GGUCCaCCg -3' miRNA: 3'- aCAaGUcUCGUGGGUGCaaCUAGG-GG- -5' |
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| 17606 | 3' | -52.9 | NC_004466.2 | + | 14833 | 0.68 | 0.677656 |
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Target: 5'- cUGcUCAG-GCAgCCCuggagaacccagaGCGUUGGUCCCg -3' miRNA: 3'- -ACaAGUCuCGU-GGG-------------UGCAACUAGGGg -5' |
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| 17606 | 3' | -52.9 | NC_004466.2 | + | 13464 | 0.73 | 0.40895 |
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Target: 5'- --aUCaAGGGCAUCCucuACGUUGAaCCCCa -3' miRNA: 3'- acaAG-UCUCGUGGG---UGCAACUaGGGG- -5' |
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| 17606 | 3' | -52.9 | NC_004466.2 | + | 16983 | 1.13 | 0.0007 |
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Target: 5'- uUGUUCAGAGCACCCACGUUGAUCCCCa -3' miRNA: 3'- -ACAAGUCUCGUGGGUGCAACUAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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