Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17606 | 5' | -52.7 | NC_004466.2 | + | 19999 | 0.66 | 0.792699 |
Target: 5'- --uGGAACCgacGGGGGU-CUACCCGg -3' miRNA: 3'- cuuUCUUGGauaCUCCCGuGGUGGGU- -5' |
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17606 | 5' | -52.7 | NC_004466.2 | + | 23792 | 0.66 | 0.792699 |
Target: 5'- -cAAGAcCCUA-GAGGcaACCACCCAg -3' miRNA: 3'- cuUUCUuGGAUaCUCCcgUGGUGGGU- -5' |
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17606 | 5' | -52.7 | NC_004466.2 | + | 14532 | 0.66 | 0.792699 |
Target: 5'- aGGAGAGCCgc-GAGuGCugcuCCGCCCAu -3' miRNA: 3'- cUUUCUUGGauaCUCcCGu---GGUGGGU- -5' |
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17606 | 5' | -52.7 | NC_004466.2 | + | 17248 | 0.67 | 0.72866 |
Target: 5'- --uAGGGCCUGUu-GGGCACCagagguaacACCCu -3' miRNA: 3'- cuuUCUUGGAUAcuCCCGUGG---------UGGGu -5' |
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17606 | 5' | -52.7 | NC_004466.2 | + | 24687 | 0.67 | 0.702952 |
Target: 5'- -uAAGAAUCUcuucccaaugcuugGUGAGGGCAaaugcuUCACCCu -3' miRNA: 3'- cuUUCUUGGA--------------UACUCCCGU------GGUGGGu -5' |
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17606 | 5' | -52.7 | NC_004466.2 | + | 40767 | 0.68 | 0.660823 |
Target: 5'- aGGGAGAagACC-AUGAGGGCcUCGCCg- -3' miRNA: 3'- -CUUUCU--UGGaUACUCCCGuGGUGGgu -5' |
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17606 | 5' | -52.7 | NC_004466.2 | + | 21798 | 0.69 | 0.603361 |
Target: 5'- aGGGGGugCUGcUGAGGGUAaaGCCCGa -3' miRNA: 3'- cUUUCUugGAU-ACUCCCGUggUGGGU- -5' |
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17606 | 5' | -52.7 | NC_004466.2 | + | 28432 | 0.7 | 0.557804 |
Target: 5'- cGAAGGuAAUCccc-AGGGCACCACCCc -3' miRNA: 3'- -CUUUC-UUGGauacUCCCGUGGUGGGu -5' |
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17606 | 5' | -52.7 | NC_004466.2 | + | 14280 | 0.7 | 0.557804 |
Target: 5'- cGAAGAguGCUcccaGGGaGGCACCACCCAg -3' miRNA: 3'- cUUUCU--UGGaua-CUC-CCGUGGUGGGU- -5' |
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17606 | 5' | -52.7 | NC_004466.2 | + | 16946 | 1.08 | 0.001341 |
Target: 5'- gGAAAGAACCUAUGAGGGCACCACCCAu -3' miRNA: 3'- -CUUUCUUGGAUACUCCCGUGGUGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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